sinI
168
antagonist of [protein|5A6FBAE6553343092862CB79E150F934978C32A9|sinR], drives [protein|920F91E748EE079FF864011D9052B073567C41E4|slrR] from the [protein|920F91E748EE079FF864011D9052B073567C41E4|slrR](LOW) to the [protein|920F91E748EE079FF864011D9052B073567C41E4|slrR](HIGH) state
locus
BSU_24600
Molecular weight
6.47 kDa
pI
6.33
function
control of [wiki|biofilm formation]
product
antagonist of [protein|5A6FBAE6553343092862CB79E150F934978C32A9|sinR]
essential
no
synonyms
sinI
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms
This gene is a member of the following regulons
Gene
Phenotypes of a mutant
altered cell death pattern in colonies [Pubmed|23012477]
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The protein
[wiki|Domains]
[wiki|Sin domain] (aa 2-40) (according to UniProt)
Structure
[PDB|5TMX] [pubmed|31493408]
[PDB|1B0N] (complex [protein|5A6FBAE6553343092862CB79E150F934978C32A9|sinR]-[protein|24D6A7DDCB25B99FBB670E9203F826596916B35C|sinI]) [Pubmed|9799632]
[AF|P23308]
Paralogous protein(s)
[protein|C17C296F3EB2E106C380E3B5D784F3FA63F4C9B7|slrA]
Expression and Regulation
Operons
genes
[gene|24D6A7DDCB25B99FBB670E9203F826596916B35C|sinI]-[gene|5A6FBAE6553343092862CB79E150F934978C32A9|sinR]
description
[Pubmed|3125149]
regulation
repressed during exponential growth ([protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC]) [http://www.ncbi.nlm.nih.gov/sites/entrez/1906467,11751836 PubMed]
regulatory mechanism
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: activation, in a portion of cells [Pubmed|11751836], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
[protein|5A6FBAE6553343092862CB79E150F934978C32A9|sinR]: repression, [Pubmed|1664536], in [regulon|protein:5A6FBAE6553343092862CB79E150F934978C32A9|sinR regulon]
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [PubMed|7635837,11751836], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
[protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC]: repression, [PubMed|1906467,11751836], in [regulon|protein:F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|3125149], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
the mRNA is stabilized upon depletion of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]
Biological materials
Mutant
GP959 (spc), available in [wiki|Jörg Stülke]'s lab
DS91 (spc) NCIB3610 derivate, available in [wiki|Jörg Stülke]'s lab
1S98 ( ''sinI''::''kan''), [Pubmed|11466285], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S98&Search=1S98 BGSC]
GP1663 (''[gene|22BEB39F735DAAF71F1B382CD453A9C2D4CC9FA4|yqhG]-[gene|24D6A7DDCB25B99FBB670E9203F826596916B35C|sinI]-[gene|5A6FBAE6553343092862CB79E150F934978C32A9|sinR]-[gene|BF97457E986656E4A9FE7A858F5BDF1759850D5C|tasA]''), available in [wiki|Jörg Stülke]'s lab
BKE24600 ([gene|24D6A7DDCB25B99FBB670E9203F826596916B35C|sinI]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24600 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCAGTTTCTCCTCCTAA, downstream forward: _UP4_TGAATGTGCTATAATATCAC
BKK24600 ([gene|24D6A7DDCB25B99FBB670E9203F826596916B35C|sinI]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24600 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCAGTTTCTCCTCCTAA, downstream forward: _UP4_TGAATGTGCTATAATATCAC
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-12-14 05:03:13
Author of last update: Jstuelk