tnrA
168
transcriptional pleiotropic regulator ([wiki|MerR family]) involved in global nitrogen regulation
locus
BSU_13310
Molecular weight
12.99 kDa
pI
10.23
function
regulation of nitrogen assimilation
product
transcription activator/ repressor ([wiki|MerR family])
essential
no
synonyms
tnrA, scgR
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0789 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,397,411 1,397,743
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The protein
Protein family
[wiki|MerR family]
[wiki|Domains]
[wiki|HTH merR-type domain] (aa 13-81) (according to UniProt)
Structure
[PDB|4R22] (the TnrA-DNA complex) [Pubmed|25691471]
[PDB|4S0R] (the [protein|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA]-[protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA] complex) [pubmed|25691471]
[AF|Q45666]
Effectors of protein activity
feedback-inhibited [protein|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA] prevents [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA] from DNA binding
[wiki|Localization]
membrane-associated via [protein|796BD46066B1E6147048664FA78679B42FA9D628|nrgA]-[protein|EC7C2A0B5A9A30B2FAA0CF9EEB9830CE0E6F2219|nrgB] under conditions of poor nitrogen supply [Pubmed|21435182]
Expression and Regulation
Operons
genes
[gene|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]
description
[pubmed|22383849]
regulation
expressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]) [Pubmed|10671441,16547045]
regulatory mechanism
[protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: activation, [PubMed|10671441,16547045], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
[protein|641C4BDD9702804642E1753A9C779E80FABB3919|glnR]: repression, in [regulon|protein:641C4BDD9702804642E1753A9C779E80FABB3919|glnR regulon]
Biological materials
Mutant
GP252 (in frame deletion), available in [wiki|Jörg Stülke]'s lab
BKE13310 (''tnrA''::''ermC'') (available at the [http://bgsc.org/ BGSC] and in [wiki|Jörg Stülke]'s lab) [pubmed|28189581]
GP2262 (''tnrA''::''ermC'') (available in [wiki|Jörg Stülke]'s lab)
1A851 ( ''tnrA''::''erm''), [Pubmed|8799114], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A851&Search=1A851 BGSC]
1A851 ( ''tnrA''::''erm''), [Pubmed|8799114], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A851&Search=1A851 BGSC]
BKE13310 ([gene|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTCCACCCCTGGATG, downstream forward: _UP4_TAAATAATAAAAGTCCGGCT
BKK13310 ([gene|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTCCACCCCTGGATG, downstream forward: _UP4_TAAATAATAAAAGTCCGGCT
Expression vectors
for expression, purification in ''E. coli'' with N-terminal His-tag, in [wiki|pWH844]: pGP171 available in [wiki|Jörg Stülke]'s lab
pGP229 (N-terminal Strep-tag, for [wiki|SPINE], expression in ''B. subtilis'', in [wiki|pGP380]), available in [wiki|Jörg Stülke]'s lab
pGP3818 (expression of [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA] in [wiki|pBQ200]), available in [wiki|Jörg Stülke]'s lab
Antibody
available in the [wiki|Karl Forchhammer] lab
labs
[wiki|Susan Fisher], Boston, USA [http://www.bumc.bu.edu/microbiology/research/susan-h-fisher-phd/ homepage]
[wiki|Maria Schumacher], Durham, NC, USA [http://www.dukeschumacherlab.com/index.html homepage]
References
Reviews
Other original publications
[Pubmed|]
The [wiki|TnrA regulon]
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Time of last update: 2024-12-14 04:58:13
Author of last update: MBenda