NasB

• Description: nitrate reductase (electron transfer subunit)
  
  

Gene name	nasB
Synonyms	nasBA
Essential	no
Product	nitrate reductase (electron transfer subunit)
Function	nitrate respiration
MW, pI	84 kDa, 5.483
Gene length, protein length	2310 bp, 770 aa
Immediate neighbours	nasC, nasA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU03320

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): NasD

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: NasB-NasC

• Localization:

Database entries

• Structure:

• UniProt: P42433

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: nasB-nasC-nasD-nasE-nasF PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • repressed unless NO is present under anaerobic conditions (NsrR) PubMed

• Regulatory mechanism:
  • TnrA: transcription activation PubMed
  • NsrR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: