Spo0B

• Description: sporulation initiation phosphotransferase of the phosphorelay
  
  

Gene name	spo0B
Synonyms	spo0D
Essential	no
Product	sporulation initiation phosphotransferase of the phosphorelay
Function	initiation of sporulation
Function and regulation of this protein in SubtiPathways: Phosphorelay
MW, pI	22 kDa, 4.963
Gene length, protein length	576 bp, 192 aa
Immediate neighbours	obg, rpmA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU27930

Phenotypes of a mutant

Arrest of sporulation at stage 0 (initiation) PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: Spo0B-Spo0F, Spo0B-Spo0A

• Localization:

Database entries

• Structure: 1F51 (complex with Spo0F), 1IXM

• UniProt: P06535

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: spo0B-obg PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • spo0B12, point mutation 85C>T (=R29W), available in BGSC 1S54
  • spo0B136, amber mutation 103A>T (=K35X), available in BGSC 1S16
  • spo0B580ts, point mutation 193G>A (=E65K), available in BGSC 1S90
  • spo0B581ts, point mutation 461G>A (=G154D), available in BGSC 1S91

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: