SigD

• Description: RNA polymerase sigma factor SigD
  
  

Gene name	sigD
Synonyms	flaB
Essential	no
Product	RNA polymerase sigma factor SigD
Function	regulation of flagella, motility, chemotaxis and autolysis
MW, pI	29 kDa, 5.167
Gene length, protein length	762 bp, 254 aa
Immediate neighbours	cheD, swrB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU16470

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

transcription, sigma factors and their control, motility and chemotaxis

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: sigma-70 factor family (according to Swiss-Prot)

• Paralogous protein(s):

Genes/ operons controlled by SigD

dltA-dltB-dltC-dltD-dltE, cheV, degR, epr, hag, lytA-lytB-lytC, lytD, lytF, tlpA-mcpA, mcpB, mcpC, motA-motB, nfrA-ywcH

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: interaction with FlgM inhibits SigD activity PubMed

• Interactions: SigD-FlgM PubMed, SigD-(RpoB-RpoC)

• Localization:

Database entries

• Structure:

• UniProt: P10726

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-ylxG-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-ylxH-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed
  • ylxF-fliK-ylxG-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-ylxH-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed
  • sigD-swrB PubMed

• Sigma factor:
  • flgB: SigA PubMed, SigD PubMed
  • ylxF: SigD PubMed
  • sigD: SigA PubMed

• Regulation:
  • flgB promoter: repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed
  • CodY: transcription repression PubMed
  • DegU (non-phosphorylated): transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody:

References

Reviews

Jan-Willem Veening, Oscar P Kuipers
Gene position within a long transcript as a determinant for stochastic switching in bacteria.
Mol Microbiol: 2010, 76(2);269-72
[PubMed:20233302] [WorldCat.org] [DOI] (I p)

Original publications

Additional publications: PubMed