Difference between revisions of "CshA"
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=== Additional information=== | === Additional information=== | ||
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=The protein= | =The protein= | ||
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* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
| − | * '''Interactions:''' [[CshA]]-[[CshA]] {{PubMed|20572937}} | + | * '''[[Interactions]]:''' part of the [[RNA degradosome]] |
| + | ** [[CshA]]-[[CshA]] {{PubMed|20572937}} | ||
| + | ** [[CshA]]-[[CspB]] [http://www.ncbi.nlm.nih.gov/sites/entrez/16352840 PubMed] | ||
| + | ** [[CshA]]-[[PnpA]] {{PubMed|20572937}}, [[CshA]]-[[Rny]] {{PubMed|20572937}}, [[CshA]]-[[RnjA]] {{PubMed|20572937}}, [[CshA]]-[[Eno]] {{PubMed|20572937}}, [[CshA]]-[[PfkA]] {{PubMed|20572937}} | ||
| + | ** [[DeaD]]-[[CshA]] {{PubMed|20572937}} | ||
* '''Localization:''' cytoplasma, colocalizes with the ribosomes [http://www.ncbi.nlm.nih.gov/sites/entrez/16352840 PubMed], cell membrane {{PubMed|20572937}} | * '''Localization:''' cytoplasma, colocalizes with the ribosomes [http://www.ncbi.nlm.nih.gov/sites/entrez/16352840 PubMed], cell membrane {{PubMed|20572937}} | ||
Revision as of 08:07, 6 March 2011
- Description: DEAD-box RNA helicase, important for adaptation to low temperatures
| Gene name | cshA |
| Synonyms | ydbR |
| Essential | no |
| Product | DEAD-box RNA helicase |
| Function | RNA helicase |
| MW, pI | 57 kDa, 9.89 |
| Gene length, protein length | 1533 bp, 511 aa |
| Immediate neighbours | murF, ydbS |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
Categories containing this gene/protein
DEAD-box RNA helicases, cold stress proteins, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU04580
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: RNA helicase
- Protein family: helicase C-terminal domain (according to Swiss-Prot) DEAD-box RNA helicase
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions: part of the RNA degradosome
Database entries
- Structure:
- UniProt: P96614
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP1035 (aphA3), available in Stülke lab
- Expression vector:
- for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, in pGP382: pGP1387, available in Stülke lab
- for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from the native chromomsomal site: GP1026, available in Stülke lab
- for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP1386, available in Stülke lab
- lacZ fusion:
- GFP fusion: pGP1369 for chromosomal expression of CshA-YFP, available in Stülke lab
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
Mohamed Marahiel, Marburg University, Germany homepage
Your additional remarks
References
Additional publications: PubMed
