SigE

• Description: RNA polymerase sporulation mother cell-specific (early) sigma factor SigE
  
  

Gene name	sigE
Synonyms	spoIIGB
Essential	no
Product	RNA polymerase sporulation mother cell-specific (early) sigma factor SigE
Function	transcription of sporulation genes (early mother cell)
Interactions involving this protein in SubtInteract: SigE
Metabolic function and regulation of this protein in SubtiPathways: Alternative nitrogen sources
MW, pI	27 kDa, 9.212
Gene length, protein length	717 bp, 239 aa
Immediate neighbours	spoIIGA, sigG
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

transcription, sigma factors and their control

This gene is a member of the following regulons

SinR regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU15320

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: RNA polymerase sigma factor, active in the mother cell

• Protein family: sigma-70 factor family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SigE-(RpoB-RpoC)
  • SpoIIGA-SigE (Processing) PubMed

• Localization:

Database entries

• Structure:

• UniProt: P06222

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: spoIIGA-sigE-sigG PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • repression by SinR
  • Spo0A: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Bill Haldenwang, San Antonio, USA
• Charles Moran, Emory University, NC, USA homepage

References

Reviews

Michiel J L de Hoon, Patrick Eichenberger, Dennis Vitkup
Hierarchical evolution of the bacterial sporulation network.
Curr Biol: 2010, 20(17);R735-45
[PubMed:20833318] [WorldCat.org] [DOI] (I p)

The SigE regulon

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)

Andrea Feucht, Louise Evans, Jeff Errington
Identification of sporulation genes by genome-wide analysis of the sigmaE regulon of Bacillus subtilis.
Microbiology (Reading): 2003, 149(Pt 10);3023-3034
[PubMed:14523133] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Shane T Jensen, Erin M Conlon, Christiaan van Ooij, Jessica Silvaggi, José Eduardo González-Pastor, Masaya Fujita, Sigal Ben-Yehuda, Patrick Stragier, Jun S Liu, Richard Losick
The sigmaE regulon and the identification of additional sporulation genes in Bacillus subtilis.
J Mol Biol: 2003, 327(5);945-72
[PubMed:12662922] [WorldCat.org] [DOI] (P p)

Original Publications

Additional publications: PubMed