AlkA

Gene name	alkA
Synonyms
Essential	no
Product	DNA-3-methyladenine glycosylase
Function	adaptive response to alkylative DNA damage
Gene expression levels in SubtiExpress: alkA
MW, pI	35 kDa, 8.147
Gene length, protein length	909 bp, 303 aa
Immediate neighbours	ybbU, adaA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination

This gene is a member of the following regulons

AdaA regulon

The gene

Basic information

• Locus tag: BSU01800

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine (according to Swiss-Prot)

• Protein family: alkylbase DNA glycosidase alkA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P37878

• KEGG entry: [3]

• E.C. number: 3.2.2.21

Expression and regulation

• Operon: alkA PubMed

• Expression browser: alkA PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism: AdaA: positive regulation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

F Morohoshi, K Hayashi, N Munkata
Bacillus subtilis alkA gene encoding inducible 3-methyladenine DNA glycosylase is adjacent to the ada operon.
J Bacteriol: 1993, 175(18);6010-7
[PubMed:8376346] [WorldCat.org] [DOI] (P p)