SrfAA

• Description: surfactin synthetase / competence
  
  

Gene name	srfAA
Synonyms	comL
Essential	no
Product	surfactin synthetase / competence
Function	antibiotic synthesis
Gene expression levels in SubtiExpress: srfAA
MW, pI	401 kDa, 4.871
Gene length, protein length	10764 bp, 3588 aa
Immediate neighbours	hxlR, srfAB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

miscellaneous metabolic pathways, biosynthesis of antibacterial compounds, membrane proteins, phosphoproteins

This gene is a member of the following regulons

Abh regulon, CodY regulon, ComA regulon, PerR regulon, Spx regulon

The gene

Basic information

• Locus tag: BSU03480

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

• A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Ser-1006 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: membrane PubMed

Database entries

• Structure:

• UniProt: P27206

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: srfAA-srfAB-comS-srfAC-srfAD PubMed

• Expression browser: srfAA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • repressed during hydrogen peroxide stress (PerR) PubMed
  • expressed at high cell density (ComA) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed
  • ComA: transcription activation PubMed
  • PerR: transcription activation PubMed
  • Spx: transcription repression PubMed
  • Abh: transcription repressionPubMed

• Additional information:
  • A ncRNA is predicted between hxlR and srfAA PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Original publications

Additional publications: PubMed