LytC

• Description: N-acetylmuramoyl-L-alanine amidase, required for flagellar function PubMed
  
  

Gene name	lytC
Synonyms	cwlB
Essential	no
Product	N-acetylmuramoyl-L-alanine amidase
Function	major autolysin, cell separation, wall turnover lysis, motility and general cell lysis induced by sodium azide
Gene expression levels in SubtiExpress: lytC lysis, motility and general cell lysis induced by sodium azide
MW, pI	52 kDa, 10.108
Gene length, protein length	1488 bp, 496 aa
Immediate neighbours	tuaA, lytB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall degradation/ turnover, motility and chemotaxis

This gene is a member of the following regulons

SigD regulon, SlrR regulon, YvrHb regulon

The gene

Basic information

• Locus tag: BSU35620

Phenotypes of a mutant

• impaired in motility, the phenotype is suppressed by mutations in lonA or smiA PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to Swiss-Prot)

• Protein family: N-acetylmuramoyl-L-alanine amidase 3 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: contains an amidase_3 domain (like CwlC, CwlD, YqiI, YrvJ)

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • secreted (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: Q02114

• KEGG entry: [3]

• E.C. number: 3.5.1.28

Expression and regulation

• Operon: lytA-lytB-lytC PubMed

• Expression browser: lytC PubMed

• Sigma factor: SigA PubMed, SigD PubMed

• Regulation:

• Regulatory mechanism:
  • YvrHb: transcription repression PubMed
  • SlrR (in complex with SinR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A789 ( lytC::kan), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: