Difference between revisions of "McsB"

From SubtiWiki
Jump to: navigation, search
(→‎Labs working on this gene/protein)
Line 14: Line 14:
 
|style="background:#ABCDEF;" align="center"|'''Function''' || control of [[CtsR]] activity
 
|style="background:#ABCDEF;" align="center"|'''Function''' || control of [[CtsR]] activity
 
|-
 
|-
−
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU00850 mcsB]
+
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00850 mcsB]
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/McsB McsB]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/McsB McsB]
Line 26: Line 26:
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mcsA]]'', ''[[clpC]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mcsA]]'', ''[[clpC]]''
 
|-
 
|-
−
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB11861&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU00850 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU00850 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU00850 Advanced_DNA]
 
|-
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:mcsB_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:mcsB_context.gif]]

Revision as of 11:57, 13 May 2013

Gene name mcsB
Synonyms yacI
Essential no
Product protein arginine kinase
Function control of CtsR activity
Gene expression levels in SubtiExpress: mcsB
Interactions involving this protein in SubtInteract: McsB
Metabolic function and regulation of this protein in SubtiPathways:
Stress
MW, pI 40 kDa, 5.068
Gene length, protein length 1089 bp, 363 aa
Immediate neighbours mcsA, clpC
Sequences Protein DNA Advanced_DNA
Genetic context
McsB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
McsB expression.png















Categories containing this gene/protein

protein modification, transcription factors and their control, sporulation proteins, general stress proteins (controlled by SigB), heat shock proteins

This gene is a member of the following regulons

CtsR regulon, SigB regulon, SigF regulon

The gene

Basic information

  • Locus tag: BSU00850

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: targets non-functional CtsR for degradation by ClpP/ClpC PubMed
  • Protein family: ATP:guanido phosphotransferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
    • autophosphorylation on specific arginine residues PubMed, dephosphorylation by YwlE PubMed
    • autophosphorylation stimulates McsB activity as adaptor protein for ClpC PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
    • mcsB::aphA3 availbale from the Gerth lab
    • mcsBC167S::spec available from the Gerth lab
    • GP1457 (mcsB::aphA3), available in Stülke lab
    • BP69 (spc), available in Fabian Commichau's lab
  • Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody: available in Gerth lab

Labs working on this gene/protein

Your additional remarks

References

Reviews

Additional reviews: PubMed

Aurelia Battesti, Susan Gottesman
Roles of adaptor proteins in regulation of bacterial proteolysis.
Curr Opin Microbiol: 2013, 16(2);140-7
[PubMed:23375660] [WorldCat.org] [DOI] (I p)

Original Publications

Additional publications: PubMed

Retrieved from "http://subtiwiki.uni-goettingen.de/wiki//index.php?title=McsB&oldid=179911"