Difference between revisions of "CotC"

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(References)
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|style="background:#ABCDEF;" align="center"|'''Function''' || resistance of the spore
 
|style="background:#ABCDEF;" align="center"|'''Function''' || resistance of the spore
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU17700 cotC]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU17700 cotC]
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/CotC CotC]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/CotC CotC]
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ynzK]]'', ''[[tatAC]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ynzK]]'', ''[[tatAC]]''
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13654&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU17700 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU17700 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU17700 Advanced_DNA]
 
|-
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:cotC_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:cotC_context.gif]]

Revision as of 12:55, 13 May 2013

  • Description: spore coat protein (outer)

Gene name cotC
Synonyms
Essential no
Product spore coat protein (outer)
Function resistance of the spore
Gene expression levels in SubtiExpress: cotC
Interactions involving this protein in SubtInteract: CotC
MW, pI 14 kDa, 9.558
Gene length, protein length 354 bp, 118 aa
Immediate neighbours ynzK, tatAC
Sequences Protein DNA Advanced_DNA
Genetic context
CotC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CotC expression.png















Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

GerE regulon, SigK regulon, SpoIIID regulon

The gene

Basic information

  • Locus tag: BSU17700

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:
    • outer spore coat, more abundant at the mother cell-distal pole of the forespore PubMed
    • the CotC-CotU complex assembles around the forming spore PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: cotC (according to DBTBS)
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Application of CotZ for protein surface display

Additional publications: PubMed

Rachele Isticato, Donatella Scotto Di Mase, Emilia M F Mauriello, Maurilio De Felice, Ezio Ricca
Amino terminal fusion of heterologous proteins to CotC increases display efficiencies in the Bacillus subtilis spore system.
Biotechniques: 2007, 42(2);151-2, 154, 156
[PubMed:17373477] [WorldCat.org] [DOI] (P p)