Difference between revisions of "PhrF"
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|style="background:#ABCDEF;" align="center"|'''Function''' || control of [[ComA]] activity | |style="background:#ABCDEF;" align="center"|'''Function''' || control of [[ComA]] activity | ||
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU37470 phrF] |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/PhrF PhrF] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/PhrF PhrF] | ||
| Line 24: | Line 24: | ||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rapF]]'', ''[[ywhH]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rapF]]'', ''[[ywhH]]'' | ||
|- | |- | ||
| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU37470 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU37470 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU37470 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:phrF_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:phrF_context.gif]] | ||
Revision as of 14:07, 13 May 2013
- Description: response regulator aspartate phosphatase (RapF) regulator, antagonizes the RapF-ComA interaction
| Gene name | phrF |
| Synonyms | ywhI |
| Essential | no |
| Product | phosphatase (RapF) regulator |
| Function | control of ComA activity |
| Gene expression levels in SubtiExpress: phrF | |
| Interactions involving this protein in SubtInteract: PhrF | |
| MW, pI | 4 kDa, 10.237 |
| Gene length, protein length | 117 bp, 39 aa |
| Immediate neighbours | rapF, ywhH |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
genetic competence, transcription factors and their control, quorum sensing, short peptides
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU37470
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: binds to RapF, this results in the inability of RapF to interact with ComA PubMed
- Protein family: phr family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P71001
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- PhrF accumulates at high cell density
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Melinda D Baker, Matthew B Neiditch
Structural basis of response regulator inhibition by a bacterial anti-activator protein.
PLoS Biol: 2011, 9(12);e1001226
[PubMed:22215984]
[WorldCat.org]
[DOI]
(I p)
Jennifer M Auchtung, Catherine A Lee, Alan D Grossman
Modulation of the ComA-dependent quorum response in Bacillus subtilis by multiple Rap proteins and Phr peptides.
J Bacteriol: 2006, 188(14);5273-85
[PubMed:16816200]
[WorldCat.org]
[DOI]
(P p)
Natalia Comella, Alan D Grossman
Conservation of genes and processes controlled by the quorum response in bacteria: characterization of genes controlled by the quorum-sensing transcription factor ComA in Bacillus subtilis.
Mol Microbiol: 2005, 57(4);1159-74
[PubMed:16091051]
[WorldCat.org]
[DOI]
(P p)
Cristina Bongiorni, Shu Ishikawa, Sophie Stephenson, Naotake Ogasawara, Marta Perego
Synergistic regulation of competence development in Bacillus subtilis by two Rap-Phr systems.
J Bacteriol: 2005, 187(13);4353-61
[PubMed:15968044]
[WorldCat.org]
[DOI]
(P p)
R S McQuade, N Comella, A D Grossman
Control of a family of phosphatase regulatory genes (phr) by the alternate sigma factor sigma-H of Bacillus subtilis.
J Bacteriol: 2001, 183(16);4905-9
[PubMed:11466295]
[WorldCat.org]
[DOI]
(P p)
