Difference between revisions of "GuaC"

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Revision as of 14:28, 16 May 2013

  • Description: GMP reductase

Gene name guaC
Synonyms yumD
Essential no
Product GMP reductase
Function purine salvage and interconversion
Gene expression levels in SubtiExpress: guaC
Metabolic function and regulation of this protein in SubtiPathways:
Purine synthesis, Nucleotides (regulation)
MW, pI 35 kDa, 6.078
Gene length, protein length 978 bp, 326 aa
Immediate neighbours yuzG, paiB
Sequences Protein DNA DNA_with_flanks
Genetic context
GuaC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GuaC expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides

This gene is a member of the following regulons

CodY regulon, PurR regulon

The gene

Basic information

  • Locus tag: BSU32130

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH (according to Swiss-Prot)
  • Protein family: GuaC type 2 subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1YPF (from Bacillus anthracis, 84% identity, 91% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • repressed in the presence of purine nucleotides (PurR) PubMed
    • activated during growth in the presence of branched chain amino acids (CodY) PubMed
    • expression activated by glucose (3.3 fold) PubMed
  • Regulatory mechanism:
    • CodY: transcription activation PubMed
    • PurR: transcription repression (molecular inducer: PRPP) PubMed
  • Additional information:
    • the mRNA is substantially stabilized upon depletion of RNase Y (the half-life of the mRNA increases from 3 to 20 min) PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J  
RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y. 
Mol Microbiol. 2011 81(6): 1459-1473. 
PubMed:21815947