PhrC

• Description: response regulator aspartate phosphatase (RapC) regulator / competence and sporulation stimulating factor (CSF)
  
  

Gene name	phrC
Synonyms
Essential	no
Product	phosphatase (RapC) regulator / competence and sporulation stimulating factor (CSF)
Function	control of ComA activity
Gene expression levels in SubtiExpress: phrC
Interactions involving this protein in SubtInteract: PhrC
MW, pI	4 kDa, 8.16
Gene length, protein length	120 bp, 40 aa
Immediate neighbours	rapC, yczM
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

genetic competence, transcription factors and their control, quorum sensing, short peptides

This gene is a member of the following regulons

CcpA regulon, CodY regulon, ComA regulon, SigH regulon

The gene

Basic information

• Locus tag: BSU03780

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: binds to RapC, this results in the inability of RapC to interact with ComA PubMed

• Protein family: phr family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • RapC-PhrC PubMed
  • RapJ-PhrC PubMed

• Localization:

Database entries

• Structure:
  • 4GYO (complex RapJ-PhrC) PubMed

• UniProt: P94416

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • rapC-phrC PubMed
  • phrC PubMed

• Expression browser: phrC PubMed

• Sigma factor:
  • rapC: SigA PubMed
  • phrC: SigH PubMed

• Regulation:
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • repressed by glucose (4.5-fold) (CcpA) PubMed
  • expressed at high cell density (ComA) PubMed

• Regulatory mechanism:
  • ComA: transcription activation (ComA) PubMed
  • CodY: transcription repression PubMed
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Elizabeth Anne Shank, Roberto Kolter
Extracellular signaling and multicellularity in Bacillus subtilis.
Curr Opin Microbiol: 2011, 14(6);741-7
[PubMed:22024380] [WorldCat.org] [DOI] (I p)