CtpB

• Description: carboxy-terminal processing serine protease, cleaves SpoIVFA, this results in processing of pro-SigK
  
  

Gene name	ctpB
Synonyms	yvjB
Essential	no
Product	carboxy-terminal processing serine protease, cleaves SpoIVFA
Function	control of SigK activation
Gene expression levels in SubtiExpress: ctpB
Interactions involving this protein in SubtInteract: CtpB
MW, pI	52 kDa, 8.641
Gene length, protein length	1440 bp, 480 aa
Immediate neighbours	swrAA/2, ftsX
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

proteolysis, sigma factors and their control, sporulation proteins

This gene is a member of the following regulons

SigE regulon, SigG regulon

The gene

Basic information

• Locus tag: BSU35240

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: PDZ protease PubMed

• Paralogous protein(s): CtpA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:
  • substrate binding of CtpB induces opening of the PDZ gate and protease activation PubMed
  • CtpB acts in concert with a second signaling protease, SpoIVB PubMed

• Interactions:
  • forms a dimeric ring PubMed
  • CtpB-SpoIVFA PubMed

• Localization:
  • intracellular space between the mother cell and the forespore PubMed

Database entries

• Structure:
  • 4C2C PubMed
  • forms a ring-like scaffold with two narrow tunnels for substrate binding PubMed

• UniProt: O35002

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ctpB (according to DBTBS)

• Expression browser: ctpB PubMed

• Sigma factor: SigE PubMed, SigG PubMed

• Regulation: expressed during sporulation (SigE, SigG) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1S129 ( ctpB::tet), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: