Difference between revisions of "Sfp/1"
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=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU03569&redirect=T BSU03569] | ||
* '''DBTBS entry:''' no entry | * '''DBTBS entry:''' no entry | ||
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=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU03569&redirect=T BSU03569] | ||
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1QR0 1QR0] (complex with CoA) | * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1QR0 1QR0] (complex with CoA) | ||
Revision as of 12:58, 2 April 2014
- Description: 4'-phosphopantetheinyl transferase (surfactin synthetase-activating enzyme), inactive pseudogene in strain 168
| Gene name | sfp/1 |
| Synonyms | sfp |
| Essential | no |
| Product | 4'-phosphopantetheinyl transferase |
| Function | phosphopantetheinylates a serine residue in each of the seven peptidyl carrier protein domains of the first three subunits of surfactin synthetase (SrfAA-SrfAB-SrfAC) and AcpK |
| Gene expression levels in SubtiExpress: sfp/1 | |
| MW, pI | - , - |
| Gene length, protein length | 165 bp, - |
| Immediate neighbours | ycxD, sfp/2 |
| Gene sequence (+200bp) | Protein sequence |
| Caution: The sequence for this gene in SubtiList contains errors | |
Genetic context 
This image was kindly provided by SubtiList
| |
| Expression at a glance PubMed 500px | |
Contents
Categories containing this gene/protein
miscellaneous metabolic pathways, biofilm formation, biosynthesis of antibacterial compounds, pseudogenes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU03569
Phenotypes of a mutant
No swarming motility on B medium. PubMed
Database entries
- BsubCyc: BSU03569
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
- This gene is a pseudogene in B. subtilis 168 due to a mutation that resulted in an internal frameshift. The second part of the pseudogene is sfp/2.
- Correction of sfp, epsC, swrAA, and degQ as well as introduction of rapP from a plasmid present in NCIB3610 results in biofilm formation in B. subtilis 168 PubMed
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: CoA + apo-[peptidyl-carrier protein] = adenosine 3',5'-bisphosphate + holo-[peptidyl-carrier protein] (according to Swiss-Prot)
- Protein family: Gsp/sfp/hetI/acpT family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- BsubCyc: BSU03569
- Structure: 1QR0 (complex with CoA)
- Swiss prot entry:
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Mohamed Marahiel, Marburg University, Germany homepage
Your additional remarks
References
Reviews
Original publications
