RpsO

• Description: ribosomal protein
  
  

Gene name	rpsO
Synonyms
Essential	yes PubMed
Product	ribosomal protein S15 (BS18)
Function	translation
Gene expression levels in SubtiExpress: rpsO
Interactions involving this protein in SubtInteract: RpsO
MW, pI	10 kDa, 10.975
Gene length, protein length	267 bp, 89 aa
Immediate neighbours	ribC, pnpA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU16680

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU16680

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ribosomal protein S15P family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • 23S rRNA-S15 PubMed
  • 16S rRNA-S15 PubMed

• Localization:

Database entries

• BsubCyc: BSU16680

• Structure: 1A32 (Geobacillus stearothermophilus)

• UniProt: P21473

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: rpsO PubMed

• Expression browser: rpsO PubMed

• Sigma factor:

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed
  • translational repression of rpsO expression upon binding of excess RpsO to a RNA switch in the untranslated region of the rpsO mRNA PubMed

• Regulatory mechanism:

• Additional information:
  • the mRNA is processed, this is initiated by RNase Y and cleavage products are subsequently subject to exonucleolytic degradation by RNases J1 and J2 and by PNPase PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Kaila Deiorio-Haggar, Jon Anthony, Michelle M Meyer
RNA structures regulating ribosomal protein biosynthesis in bacilli.
RNA Biol: 2013, 10(7);1180-4
[PubMed:23611891] [WorldCat.org] [DOI] (I p)

Genki Akanuma, Hideaki Nanamiya, Yousuke Natori, Koichi Yano, Shota Suzuki, Shuya Omata, Morio Ishizuka, Yasuhiko Sekine, Fujio Kawamura
Inactivation of ribosomal protein genes in Bacillus subtilis reveals importance of each ribosomal protein for cell proliferation and cell differentiation.
J Bacteriol: 2012, 194(22);6282-91
[PubMed:23002217] [WorldCat.org] [DOI] (I p)

Shiyi Yao, David H Bechhofer
Initiation of decay of Bacillus subtilis rpsO mRNA by endoribonuclease RNase Y.
J Bacteriol: 2010, 192(13);3279-86
[PubMed:20418391] [WorldCat.org] [DOI] (I p)

Matthew A Lauber, William E Running, James P Reilly
B. subtilis ribosomal proteins: structural homology and post-translational modifications.
J Proteome Res: 2009, 8(9);4193-206
[PubMed:19653700] [WorldCat.org] [DOI] (P p)

Shiyi Yao, David H Bechhofer
Processing and stability of inducibly expressed rpsO mRNA derivatives in Bacillus subtilis.
J Bacteriol: 2009, 191(18);5680-9
[PubMed:19633085] [WorldCat.org] [DOI] (I p)

Lincoln G Scott, James R Williamson
The binding interface between Bacillus stearothermophilus ribosomal protein S15 and its 5'-translational operator mRNA.
J Mol Biol: 2005, 351(2);280-90
[PubMed:16005889] [WorldCat.org] [DOI] (P p)

L G Scott, J R Williamson
Interaction of the Bacillus stearothermophilus ribosomal protein S15 with its 5'-translational operator mRNA.
J Mol Biol: 2001, 314(3);413-22
[PubMed:11846555] [WorldCat.org] [DOI] (P p)

G M Culver, J H Cate, G Z Yusupova, M M Yusupov, H F Noller
Identification of an RNA-protein bridge spanning the ribosomal subunit interface.
Science: 1999, 285(5436);2133-6
[PubMed:10497132] [WorldCat.org] [DOI] (P p)