Difference between revisions of "PolY2"

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|-
 
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|style="background:#ABCDEF;" align="center"|'''Function''' || UV-targeted mutagenesis
 
|style="background:#ABCDEF;" align="center"|'''Function''' || UV-targeted mutagenesis
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|-
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU23710 polY2]
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|-
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=PolY2 PolY2]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 45 kDa, 9.532   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 45 kDa, 9.532   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqjX]]'', ''[[yqzH]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqjX]]'', ''[[yqzH]]''
 
|-
 
|-
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14303&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU23710 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU23710 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU23710 DNA_with_flanks]
 
|-
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yqjW_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yqjW_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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|-
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|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=polYB_2464562_2465800_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:polY2_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU23710]]
 
|-
 
|-
 
|}
 
|}
  
 
__TOC__
 
__TOC__
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<br/><br/><br/><br/>
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<br/><br/><br/><br/>
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<br/><br/><br/><br/>
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<br/><br/>
  
−
<br/><br/>
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= [[Categories]] containing this gene/protein =
 +
{{SubtiWiki category|[[DNA repair/ recombination]]}}
 +
 
 +
= This gene is a member of the following [[regulons]] =
 +
{{SubtiWiki regulon|[[LexA regulon]]}}
  
 
=The gene=
 
=The gene=
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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU23710&redirect=T BSU23710]
  
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yqjWX.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yqjWX.html]
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= Categories containing this gene/protein =
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−
{{SubtiWiki category|[[DNA repair/ recombination]]}}
 
 
=The protein=
 
=The protein=
  
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* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
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* '''Interactions:'''  
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* '''[[SubtInteract|Interactions]]:'''
 
** [[PolY2]]-[[DnaN]] (required for untargeted mutagenesis) {{PubMed|15469515}}
 
** [[PolY2]]-[[DnaN]] (required for untargeted mutagenesis) {{PubMed|15469515}}
 
** [[PolA]]-[[PolY2]] {{PubMed|16045613}}  
 
** [[PolA]]-[[PolY2]] {{PubMed|16045613}}  
  
−
* '''Localization:'''
+
* '''[[Localization]]:'''
  
 
=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU23710&redirect=T BSU23710]
  
 
* '''Structure:'''
 
* '''Structure:'''
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* '''Operon:''' ''[[polY2]]-[[yqjX]]'' (according to [http://dbtbs.hgc.jp/COG/prom/yqjWX.html DBTBS])
 
* '''Operon:''' ''[[polY2]]-[[yqjX]]'' (according to [http://dbtbs.hgc.jp/COG/prom/yqjWX.html DBTBS])
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=polYB_2464562_2465800_-1 polY2] {{PubMed|22383849}}
  
 
* '''[[Sigma factor]]:'''  
 
* '''[[Sigma factor]]:'''  
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=Biological materials =
 
=Biological materials =
  
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* '''Mutant:'''
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* '''Mutant:''' GP1505 (''polY2''::''kan''), available in [[Stülke]] lab
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''
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=References=
 
=References=
−
 
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==Reviews==
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<pubmed>16267290,  12644484 15469515 16045613 19924481 </pubmed>
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<pubmed> 22933559 </pubmed>
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== Original publications ==
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<pubmed>16267290,  12644484 15469515 16045613 19924481 23686288 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Latest revision as of 14:08, 2 April 2014

  • Description: translesion synthesis (TLS-) DNA polymerase Y2

Gene name polY2
Synonyms yqjW
Essential no
Product translesion synthesis (TLS-) DNA polymerase Y2
Function UV-targeted mutagenesis
Gene expression levels in SubtiExpress: polY2
Interactions involving this protein in SubtInteract: PolY2
MW, pI 45 kDa, 9.532
Gene length, protein length 1236 bp, 412 aa
Immediate neighbours yqjX, yqzH
Sequences Protein DNA DNA_with_flanks
Genetic context
YqjW context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PolY2 expression.png















Categories containing this gene/protein

DNA repair/ recombination

This gene is a member of the following regulons

LexA regulon

The gene

Basic information

  • Locus tag: BSU23710

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)
  • Protein family: DNA polymerase type-Y family (according to Swiss-Prot)

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP1505 (polY2::kan), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original publications

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