Difference between revisions of "MgsR"

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* '''Description:''' transcriptional regulator of a subset of the SigB general stress regulon <br/><br/>
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* '''Description:''' transcriptional regulator of a subset of the [[SigB]] general stress regulon, required for protection against oxidative stress  <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Function''' || controls a subset of general stress genes
 
|style="background:#ABCDEF;" align="center"|'''Function''' || controls a subset of general stress genes
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|-
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU24770 mgsR]
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|-
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=MgsR MgsR]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 14 kDa, 9.28   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 14 kDa, 9.28   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[trnSL-Gln1]]'', ''[[yqgY]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[trnSL-Gln1]]'', ''[[yqgY]]''
 
|-
 
|-
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/yqgZ_nucleotide.txt    Gene sequence     (+200bp)  ]'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU24770 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU24770 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU24770 DNA_with_flanks]
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/yqgZ_protein.txt Protein sequence]'''
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|-
 
|-
 
|-
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|colspan="2" style="background:#FAF8CC;color:#FF0000" align="center" | '''Caution: The sequence for this gene in SubtiList contains errors
 
 
|-
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yqgZ_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yqgZ_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 +
|-
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|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mgsR_2564026_2564406_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:mgsR_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU24770]]
 
|-
 
|-
 
|}
 
|}
  
 
__TOC__
 
__TOC__
 +
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 +
 +
= [[Categories]] containing this gene/protein =
 +
{{SubtiWiki category|[[transcription factors and their control]]}},
 +
{{SubtiWiki category|[[general stress proteins (controlled by SigB)]]}},
 +
{{SubtiWiki category|[[resistance against oxidative and electrophile stress]]}}
  
−
<br/><br/>
+
= This gene is a member of the following [[regulons]] =
 +
{{SubtiWiki regulon|[[SigB regulon]]}},
 +
{{SubtiWiki regulon|[[MgsR regulon]]}}
  
 
=The gene=
 
=The gene=
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=== Basic information ===
 
=== Basic information ===
  
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* '''Coordinates:'''
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* '''Locus tag:''' BSU24770
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
  
 
=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU24770&redirect=T BSU24770]
  
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yqgZ.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yqgZ.html]
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=== Additional information===
 
=== Additional information===
−
 
  
 
=The protein=
 
=The protein=
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* '''Catalyzed reaction/ biological activity:''' exerts a positive or negative effect on the expression of 53 or 18 stress genes, respectively [http://www.ncbi.nlm.nih.gov/sites/entrez/18643936 PubMed]
 
* '''Catalyzed reaction/ biological activity:''' exerts a positive or negative effect on the expression of 53 or 18 stress genes, respectively [http://www.ncbi.nlm.nih.gov/sites/entrez/18643936 PubMed]
  
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* '''Protein family:''' Arsenate Reductase (ArsC) family, Spx subfamily
+
* '''Protein family:''' arsC family (according to Swiss-Prot) Arsenate Reductase (ArsC) family, Spx subfamily
  
 
* '''Paralogous protein(s):''' [[Spx]]
 
* '''Paralogous protein(s):''' [[Spx]]
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* '''Modification:'''
 
* '''Modification:'''
 +
** [[MgsR]] is subject to post-translational redox-sensitive activation by intramolecular disulfide bond formation in response to ethanol stress {{PubMed|22812682}}
  
 
* '''Cofactor(s):'''
 
* '''Cofactor(s):'''
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* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
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* '''Interactions:'''
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* '''[[SubtInteract|Interactions]]:'''  
 +
** [[MgsR]]-[[RpoA]] {{PubMed|18643936}}
  
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* '''Localization:'''
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* '''[[Localization]]:'''
  
 
=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU24770&redirect=T BSU24770]
  
 
* '''Structure:'''
 
* '''Structure:'''
  
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* '''Swiss prot entry:'''
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* '''UniProt:''' [http://www.uniprot.org/uniprot/P54503 P54503]
  
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* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU24770]
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* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU24770]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
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=Expression and regulation=
 
=Expression and regulation=
  
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* '''Operon:'''  
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* '''Operon:''' ''mgsR'' {{PubMed|18643936}}
 +
 
 +
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mgsR_2564026_2564406_-1 mgsR] {{PubMed|22383849}}
  
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* '''Sigma factor:''' [[SigB]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18643936 PubMed]
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* '''[[Sigma factor]]:''' [[SigB]] {{PubMed|18643936}}
  
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* '''Regulation:''' induced by stress ([[SigB]]) [http://www.ncbi.nlm.nih.gov/pubmed/15805528 PubMed], expressed under stress conditions [http://www.ncbi.nlm.nih.gov/sites/entrez/18643936 PubMed]
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* '''Regulation:'''  
 +
** repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]  
 +
** induced by stress ([[SigB]]) {{PubMed|15805528,18643936}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
−
 
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** MgsR: transcription activation {{PubMed|22812682}}
 
* '''Additional information:'''
 
* '''Additional information:'''
 +
** [[MgsR]] is subject to post-translational redox-sensitive activation by intramolecular disulfide bond formation in response to ethanol stress {{PubMed|22812682}}
 +
** [[MgsR]] is subject to rapid proteolytic degradation by [[ClpX]]-[[ClpP]] and [[ClpC]]-[[ClpP]] {{PubMed|22812682}}
  
 
=Biological materials =
 
=Biological materials =
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=References=
 
=References=
 +
<pubmed>18643936, 12107147 15805528 18643936,22812682 22582280</pubmed>
  
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# H&ouml;per et al. (2005) Comprehensive Characterization of the Contribution of Individual ''[[SigB]]''-Dependent General Stress Genes to Stress Resistance of ''Bacillus subtilis''. J. Bact. '''187:''' 2810-2826  [http://www.ncbi.nlm.nih.gov/pubmed/15805528 PubMed]
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[[Category:Protein-coding genes]]
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# Reder et al. (2008) The Spx paralogue MgsR (YqgZ) controls a subregulon within the general stress response of ''Bacillus subtilis''. ''Mol. Microbiol.'' '''69:''' 1104-1120. [http://www.ncbi.nlm.nih.gov/sites/entrez/18643936 PubMed]
 
−
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 

Latest revision as of 14:13, 2 April 2014

  • Description: transcriptional regulator of a subset of the SigB general stress regulon, required for protection against oxidative stress

Gene name mgsR
Synonyms yqgZ
Essential no
Product transcriptional regulator
Function controls a subset of general stress genes
Gene expression levels in SubtiExpress: mgsR
Interactions involving this protein in SubtInteract: MgsR
MW, pI 14 kDa, 9.28
Gene length, protein length 378 bp, 126 aa
Immediate neighbours trnSL-Gln1, yqgY
Sequences Protein DNA DNA_with_flanks
Genetic context
YqgZ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MgsR expression.png

















Categories containing this gene/protein

transcription factors and their control, general stress proteins (controlled by SigB), resistance against oxidative and electrophile stress

This gene is a member of the following regulons

SigB regulon, MgsR regulon

The gene

Basic information

  • Locus tag: BSU24770

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: exerts a positive or negative effect on the expression of 53 or 18 stress genes, respectively PubMed
  • Protein family: arsC family (according to Swiss-Prot) Arsenate Reductase (ArsC) family, Spx subfamily
  • Paralogous protein(s): Spx

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
    • MgsR is subject to post-translational redox-sensitive activation by intramolecular disulfide bond formation in response to ethanol stress PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
    • MgsR: transcription activation PubMed
  • Additional information:
    • MgsR is subject to post-translational redox-sensitive activation by intramolecular disulfide bond formation in response to ethanol stress PubMed
    • MgsR is subject to rapid proteolytic degradation by ClpX-ClpP and ClpC-ClpP PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

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