MetC

• Description: cystathionine beta-lyase
  
  

Gene name	metC
Synonyms	yjcJ
Essential	no
Product	cystathionine beta-lyase
Function	biosynthesis of methionine
Gene expression levels in SubtiExpress: metC
Metabolic function and regulation of this protein in SubtiPathways: metC
MW, pI	42 kDa, 5.757
Gene length, protein length	1170 bp, 390 aa
Immediate neighbours	metI, yjcK
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

S-box

The gene

Basic information

• Locus tag: BSU11880

Database entries

• BsubCyc: BSU11880

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-cystathionine + H₂O = L-homocysteine + NH₃ + pyruvate (according to Swiss-Prot)

• Protein family: trans-sulfuration enzymes family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU11880

• Structure:

• UniProt: O31632

• KEGG entry: [3]

• E.C. number: 4.4.1.8

Expression and regulation

• Operon: metI-metC PubMed

• Expression browser: metC PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by casamino acids PubMed
  • induced by methionine starvation (S-box) PubMed

• Regulatory mechanism: S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 623 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 442 PubMed

Biological materials

• Mutant:
  • 1A944 ( metC::kan), PubMed, available at BGSC
  • 1A940 ( metC::spec), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: