GpsB

• Description: required for removal of PBP1 from the cell pole after completion of cell pole maturation
  
  

Gene name	gpsB
Synonyms	ypsB
Essential	no
Product	cell division protein, member of the divisome
Function	cell division, cell elongation
Gene expression levels in SubtiExpress: gpsB
Interactions involving this protein in SubtInteract: GpsB
MW, pI	11 kDa, 5.629
Gene length, protein length	294 bp, 98 aa
Immediate neighbours	rnpB, ypsA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell division, membrane proteins, phosphoproteins

The gene

Basic information

• Locus tag: BSU22180

Phenotypes of a mutant

• synthetic lethal phenotype with a ftsA mutation PubMed; synthetic sick phenotype when combined with an ezrA mutation PubMed

Database entries

• BsubCyc: BSU22180

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): DivIVA

Extended information on the protein

• Kinetic information:

• Domains: contains a DivIVA-like N-terminal domain

• Modification: phosphorylation on (Ser-73 OR Thr-75) PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • GpsB-EzrA PubMed
  • GpsB-PonA PubMed
  • GpsB-MreC PubMed

• Localization:
  • dynamic, shuttles between lateral membrane and the divison septum PubMed, localizes to the divisome after Z-ring formation PubMed

Database entries

• BsubCyc: BSU22180

• Structure:

• UniProt: P50839

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: gpsB PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1059 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1205 PubMed

Biological materials

• Mutant:
  • 1A934 ( gpsB::kan), PubMed, available at BGSC
  • 1A970 ( gpsB::kan), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: