CpgA

• Description: GTPase, activity stimulated by ribosomes, may be involved in ribosome maturation
  
  

Gene name	cpgA
Synonyms	yloQ
Essential	no
Product	GTPase
Function	ribosome assembly, coordination of peptidoglycan deposition in the cell wall
Gene expression levels in SubtiExpress: cpgA
Metabolic function and regulation of this protein in SubtiPathways: cpgA
MW, pI	33 kDa, 4.743
Gene length, protein length	894 bp, 298 aa
Immediate neighbours	prkC, rpe
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall/ other, translation, GTP-binding proteins, phosphoproteins

The gene

Basic information

• Locus tag: BSU15780

Phenotypes of a mutant

• forms curly cells PubMed

Database entries

• BsubCyc: BSU15780

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: engC GTPase domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • in vitro phosphorylated on Thr-166 by PrkC, dephosphorylated by PrpC PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • binds the 30S subunit of the ribosome PubMed

• Localization:

Database entries

• BsubCyc: BSU15780

• Structure: 1T9H

• UniProt: O34530

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:
  • prpC-prkC-cpgA PubMed
  • cpgA PubMed

• Expression browser: cpgA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 170 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 265 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Tony Wilkinson, York University, U.K. homepage

References

Reviews

Robert A Britton
Role of GTPases in bacterial ribosome assembly.
Annu Rev Microbiol: 2009, 63;155-76
[PubMed:19575570] [WorldCat.org] [DOI] (I p)

Original publications

Frédérique Pompeo, Céline Freton, Catherine Wicker-Planquart, Christophe Grangeasse, Jean-Michel Jault, Anne Galinier
Phosphorylation of CpgA protein enhances both its GTPase activity and its affinity for ribosome and is crucial for Bacillus subtilis growth and morphology.
J Biol Chem: 2012, 287(25);20830-8
[PubMed:22544754] [WorldCat.org] [DOI] (I p)

Cédric Absalon, Michal Obuchowski, Edwige Madec, Delphine Delattre, I Barry Holland, Simone J Séror
CpgA, EF-Tu and the stressosome protein YezB are substrates of the Ser/Thr kinase/phosphatase couple, PrkC/PrpC, in Bacillus subtilis.
Microbiology (Reading): 2009, 155(Pt 3);932-943
[PubMed:19246764] [WorldCat.org] [DOI] (P p)

Ishita M Shah, Maria-Halima Laaberki, David L Popham, Jonathan Dworkin
A eukaryotic-like Ser/Thr kinase signals bacteria to exit dormancy in response to peptidoglycan fragments.
Cell: 2008, 135(3);486-96
[PubMed:18984160] [WorldCat.org] [DOI] (I p)

Cédric Absalon, Kassem Hamze, Didier Blanot, Claude Frehel, Rut Carballido-Lopez, Barry I Holland, Jean van Heijenoort, Simone J Séror
The GTPase CpgA is implicated in the deposition of the peptidoglycan sacculus in Bacillus subtilis.
J Bacteriol: 2008, 190(10);3786-90
[PubMed:18344364] [WorldCat.org] [DOI] (I p)

Alison Hunt, Joy P Rawlins, Helena B Thomaides, Jeff Errington
Functional analysis of 11 putative essential genes in Bacillus subtilis.
Microbiology (Reading): 2006, 152(Pt 10);2895-2907
[PubMed:17005971] [WorldCat.org] [DOI] (P p)

Lionel Cladière, Kassem Hamze, Edwige Madec, Vladimir M Levdikov, Anthony J Wilkinson, I Barry Holland, Simone J Séror
The GTPase, CpgA(YloQ), a putative translation factor, is implicated in morphogenesis in Bacillus subtilis.
Mol Genet Genomics: 2006, 275(4);409-20
[PubMed:16485133] [WorldCat.org] [DOI] (P p)

Tracey L Campbell, Denis M Daigle, Eric D Brown
Characterization of the Bacillus subtilis GTPase YloQ and its role in ribosome function.
Biochem J: 2005, 389(Pt 3);843-52
[PubMed:15828870] [WorldCat.org] [DOI] (I p)

Vladimir M Levdikov, Elena V Blagova, James A Brannigan, Lionel Cladière, Alfred A Antson, Michail N Isupov, Simone J Séror, Anthony J Wilkinson
The crystal structure of YloQ, a circularly permuted GTPase essential for Bacillus subtilis viability.
J Mol Biol: 2004, 340(4);767-82
[PubMed:15223319] [WorldCat.org] [DOI] (P p)

Lionel Cladière, Elena Blagova, Vladimir M Levdikov, James A Brannigan, Simone J Séror, Anthony J Wilkinson
Crystallization of YloQ, a GTPase of unknown function essential for Bacillus subtilis viability.
Acta Crystallogr D Biol Crystallogr: 2004, 60(Pt 2);329-30
[PubMed:14747714] [WorldCat.org] [DOI] (P p)