purN

purN
168

phosphoribosylglycinamide formyltransferase, irreversible

Locus
BSU_06510
Molecular weight
21.63 kDa
Isoelectric point
6.18
Protein length
Gene length
Function
purine biosynthesis
Product
phosphoribosylglycinamide formyltransferase
Essential
no
E.C.
2.1.2.2
Synonyms
purN

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0299 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
708,010  708,597
The protein
Catalyzed reaction/ biological activity
(6S)-10-formyltetrahydrofolate + N1-(5-phospho-D-ribosyl)glycinamide --> (6S)-5,6,7,8-tetrahydrofolate + H+ + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide (according to UniProt)
Protein family
GART family (single member, according to UniProt)
Structure
3P9X (PDB) (from B. halodurans, 51% identity, 70% similarity)
Paralogous protein(s)
Expression and Regulation
Operons
Description
Regulation
expression activated by glucose (4.4 fold) PubMed
Regulatory mechanism
G-box: RNA switch, (riboswitch) PubMed, in G-box
PurR: repression, (molecular inducer: PRPP) PubMed, in purR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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purEpurD

2024-11-26 09:33:54

Jstuelk

207

ace69dc80f4f087486c218727fa370a7a9630d21

EDD8EC37B0E5B38941D5827C265AB2F23CF23EA7

Biological materials
Mutant
BKE06510 (purN::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_TGATGCAAATACCGCAAACT,  downstream forward: _UP4_TTAAATAACAGAGGTGAAAA
BKK06510 (purN::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_TGATGCAAATACCGCAAACT,  downstream forward: _UP4_TTAAATAACAGAGGTGAAAA
References
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Page visits: 161560

Time of last update: 2024-12-03 21:28:13

Author of last update: Melvin.boenninger