Difference between revisions of "PGP172"
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| − | * vector for the expression of proteins in E. coli, the [[plasmid]] allows to fuse a Strep-tag to the N-terminus of the expressed protein | + | * vector for the expression of proteins in ''E. coli'', the [[plasmid]] allows to fuse a Strep-tag to the N-terminus of the expressed protein |
| − | * host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture | + | * host: ''E. coli'' BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture |
* constructed in the [[Stülke]] lab | * constructed in the [[Stülke]] lab | ||
| − | * in E. coli: ampicillin resistance | + | * in ''E. coli'': ampicillin resistance |
* based on pET3C | * based on pET3C | ||
| Line 18: | Line 18: | ||
**'''CD13:''' 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’ | **'''CD13:''' 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’ | ||
** '''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’ | ** '''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’ | ||
| + | |||
| + | * important: ''Eco''RI, although mentioned in the MCS, is not unique! | ||
<pubmed> 14717704 </pubmed> | <pubmed> 14717704 </pubmed> | ||
| + | == Return to [[Plasmids]] == | ||
Latest revision as of 14:04, 3 June 2013
- vector for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the N-terminus of the expressed protein
- host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
- constructed in the Stülke lab
- in E. coli: ampicillin resistance
- based on pET3C
- sequencing primer:
- CD13: 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
- NP20: 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
- important: EcoRI, although mentioned in the MCS, is not unique!
