ykuP

ykuP
168

flavodoxin, binds FMN, replaces ferredoxin under conditions of iron limitation

Locus
BSU_14170
Molecular weight
20.11 kDa
Isoelectric point
4.1
Protein length
Gene length
Function
electron transfer
Product
flavodoxin
Essential
no
Synonyms
ykuP

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0716 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,488,413 → 1,488,868
The protein
Protein family
flavodoxin family (with YkuN, according to UniProt)
Flavodoxin-like domain (aa 4-144) (according to UniProt)
FMN
Structure
6FSG (PDB) (from B. cereus, 46% identity) PubMed
Paralogous protein(s)
Expression and Regulation
Operons
Description
Regulation
expressed under anaerobic conditions (ResD) PubMed
induced by iron starvation (second wave) (Fur) PubMed
Regulatory mechanism
ResD: activation, PubMed, in resD regulon
Kre: repression, in kre regulon
NsrR: repression, PubMed, in nsrR regulon
Fur: repression, PubMed, in fur regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigI: sigma factor, PubMed, in sigI regulon
Additional information
the ''YkuN-YkuO-YkuP operon is strongly unregulated in a kre'' mutant PubMed
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ykuNykuP

2024-11-08 13:41:11

Jstuelk

87

57db5b9bbc0032750db2eb9bfdbed1110c747af0

E599E3DBF7B4049028BE0EE09C6A470E8B1A923E

Biological materials
Mutant
GP4732 ΔykuP::aphA3, available in Jörg Stülke's lab
MGNA-B339 (ykuP::erm), available at the NBRP B. subtilis, Japan
BKE14170 (ΔykuP::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CAATTCCTTGTTCCTCCTCT,  downstream forward: _UP4_TGATCACTCACTGGGAACTG
BKK14170 (ΔykuP::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CAATTCCTTGTTCCTCCTCT,  downstream forward: _UP4_TGATCACTCACTGGGAACTG
References
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Time of last update: 2024-12-02 16:19:24

Author of last update: Jstuelk