Difference between revisions of "FlhG"

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* '''Description:''' GTPase activating protein, activates [[FlhF]], control of flagellar basal body position <br/><br/>
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* '''Description:''' GTPase activating protein, activates [[FlhF]], activates assembly of the flagellar C ring, control of flagellar basal body position <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
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* '''Domains:'''  
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* '''[[Domains]]:'''  
  
 
* '''Modification:'''
 
* '''Modification:'''
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* '''[[SubtInteract|Interactions]]:'''  
 
* '''[[SubtInteract|Interactions]]:'''  
 +
** forms homodimers {{PubMed|25733861}}
 
** [[FlhG]]-[[FlhF]] ([[FlhG]] binds the GTP-bound homodimer of [[FlhF]]) {{PubMed|22056770}}
 
** [[FlhG]]-[[FlhF]] ([[FlhG]] binds the GTP-bound homodimer of [[FlhF]]) {{PubMed|22056770}}
 +
** [[FlhG]]-([[FliY]]-[[FliM]])-[[FliG]] {{PubMed|25733861}}
  
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* '''[[Localization]]:''' cytoplasm (Homogeneous) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]
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* '''[[Localization]]:''' cytoplasm (homogeneous) and flagellar basal body {{PubMed|25733861,16479537}}
  
 
=== Database entries ===
 
=== Database entries ===
 
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU16410&redirect=T BSU16410]
 
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU16410&redirect=T BSU16410]
  
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* '''Structure:'''
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* '''Structure:'''  
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** [http://www.rcsb.org/pdb/explore/explore.do?structureId=4RZ3 4RZ3] (from ''Geobacillus thermodenitrificans'') {{PubMed|25733861}}
 +
** [http://www.rcsb.org/pdb/explore.do?structureId=3SYN 3SYN] (complex with [[FlhF]]) {{PubMed|22056770}}
  
 
* '''UniProt:'''  
 
* '''UniProt:'''  
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=Expression and regulation=
 
=Expression and regulation=
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* '''Operon:'''  
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* '''Operon:''' part of the [[fla-che operon]]
 
** ''[[flgB]]-[[flgC]]-[[fliE]]-[[fliF]]-[[fliG]]-[[fliH]]-[[fliI]]-[[fliJ]]-[[ylxF]]-[[fliK]]-[[flgD]]-[[flgE]]-[[fliL]]-[[fliM]]-[[fliY]]-[[cheY]]-[[fliZ]]-[[fliP]]-[[fliQ]]-[[fliR]]-[[flhB]]-[[flhA]]-[[flhF]]-[[flhG]]-[[cheB]]-[[cheA]]-[[cheW]]-[[cheC]]-[[cheD]]-[[sigD]]-[[swrB]]'' {{PubMed|9657996,8157612,15175317}}
 
** ''[[flgB]]-[[flgC]]-[[fliE]]-[[fliF]]-[[fliG]]-[[fliH]]-[[fliI]]-[[fliJ]]-[[ylxF]]-[[fliK]]-[[flgD]]-[[flgE]]-[[fliL]]-[[fliM]]-[[fliY]]-[[cheY]]-[[fliZ]]-[[fliP]]-[[fliQ]]-[[fliR]]-[[flhB]]-[[flhA]]-[[flhF]]-[[flhG]]-[[cheB]]-[[cheA]]-[[cheW]]-[[cheC]]-[[cheD]]-[[sigD]]-[[swrB]]'' {{PubMed|9657996,8157612,15175317}}
 
** ''[[ylxF]]-[[fliK]]-[[flgD]]-[[flgE]]-[[fliL]]-[[fliM]]-[[fliY]]-[[cheY]]-[[fliZ]]-[[fliP]]-[[fliQ]]-[[fliR]]-[[flhB]]-[[flhA]]-[[flhF]]-[[flhG]]-[[cheB]]-[[cheA]]-[[cheW]]-[[cheC]]-[[cheD]]-[[sigD]]-[[swrB]]'' {{PubMed|20233303}}
 
** ''[[ylxF]]-[[fliK]]-[[flgD]]-[[flgE]]-[[fliL]]-[[fliM]]-[[fliY]]-[[cheY]]-[[fliZ]]-[[fliP]]-[[fliQ]]-[[fliR]]-[[flhB]]-[[flhA]]-[[flhF]]-[[flhG]]-[[cheB]]-[[cheA]]-[[cheW]]-[[cheC]]-[[cheD]]-[[sigD]]-[[swrB]]'' {{PubMed|20233303}}
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** ''[[ylxF]]'': [[SigD]] {{PubMed|20233303}}
 
** ''[[ylxF]]'': [[SigD]] {{PubMed|20233303}}
  
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* '''Regulation:'''  
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* '''Regulation:''' see [[fla-che operon]]
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** repressed under conditions that trigger sporulation ([[Spo0A]]) {{PubMed|14651647}}
 
  
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* '''Regulatory mechanism:'''  
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* '''Regulatory mechanism:''' see [[fla-che operon]]
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** [[Spo0A]]: transcription repression {{PubMed|14651647}}
 
−
** [[CodY]]: transcription repression {{PubMed|12730172}}
 
−
** [[DegU]] (non-phosphorylated): transcription activation {{PubMed|17850253}}
 
−
** [[DegU]]-P: transcription repression {{PubMed|24386445}}
 
−
** [[DegU]]-P in complex with [[SwrA]]: transcription activation {{PubMed|24386445}}
 
  
 
* '''Additional information:'''
 
* '''Additional information:'''
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=References=
 
=References=
−
 
+
==Reviews==
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<pubmed>15066026,17850253,14651647, 16479537 9657996,8157612,15175317 22056770 23190039 24386445</pubmed>
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<pubmed> 26195616,, </pubmed>
 +
==Original Publications==
 +
<pubmed>15066026,17850253,14651647, 16479537 9657996,8157612,15175317 22056770 23190039 24386445 25733861</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Latest revision as of 10:54, 24 July 2015

  • Description: GTPase activating protein, activates FlhF, activates assembly of the flagellar C ring, control of flagellar basal body position

Gene name flhG
Synonyms ylxH
Essential no
Product GTPase activating protein
Function activation of FlhF
Gene expression levels in SubtiExpress: flhG
Interactions involving this protein in SubtInteract: FlhG
MW, pI 33 kDa, 9.648
Gene length, protein length 894 bp, 298 aa
Immediate neighbours flhF, cheB
Sequences Protein DNA DNA_with_flanks
Genetic context
YlxH context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YlxH expression.png















Categories containing this gene/protein

motility and chemotaxis, resistance against toxins/ antibiotics

This gene is a member of the following regulons

CodY regulon, DegU regulon, SigD regulon, Spo0A regulon

The gene

Basic information

  • Locus tag: BSU16410

Phenotypes of a mutant

  • susceptible to acriflavine and ethidium bromide, and severe growth inhibition as surfactin concentration increased up to 100ug/ml.

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • binds FlhF and stimulates its GTPase activity PubMed
    • controls the distance between the flagellar bodies PubMed
  • Protein family: View classification (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Jan S Schuhmacher, Kai M Thormann, Gert Bange
How bacteria maintain location and number of flagella?
FEMS Microbiol Rev: 2015, 39(6);812-22
[PubMed:26195616] [WorldCat.org] [DOI] (I p)

Original Publications

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