nrdF

nrdF
168

ribonucleoside-diphosphate reductase (beta subunit)

Locus
BSU_17390
Molecular weight
38.22 kDa
Isoelectric point
4.66
Protein length
Gene length
Function
synthesis of deoxyribonucleoside triphosphates
Product
ribonucleoside-diphosphate reductase (beta subunit)
Essential
yes
E.C.
1.17.4.1
Synonyms
nrdF

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0208 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,871,089 → 1,872,078
Phenotypes of a mutant
essential PubMed
The protein
Catalyzed reaction/ biological activity
[thioredoxin]-disulfide + 2'-deoxyribonucleoside 5'-diphosphate + H2O --> [thioredoxin]-dithiol + ribonucleoside 5'-diphosphate (according to UniProt)
required for stationary-phase mutagenesis PubMed
Protein family
ribonucleoside diphosphate reductase small chain family (with YosP, according to UniProt)
dimanganic-tyrosyl radical (MnIII2-Y•) cofactor PubMed
Structure
6MW3 (PDB) (inhibitory filament formed with NrdE) PubMed
Effectors of protein activity
dATP at concentrations up to ~10 M stimulate CDP and UDP reduction, while higher concentrations inhibit this activity, inhibition by dATP is enhanced by binding of dAMP to the N-terminal domain of NrdE PubMed
Paralogous protein(s)
Expression and Regulation
Operons
Description
Regulation
expressed under anaerobic conditions (ResD) PubMed
Regulatory mechanism
ResD: activation, PubMed, in resD regulon
NrdR: repression, (predicted) PubMed, in nrdR regulon
PerR: transcription repression, PubMed, in perR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
A ncRNA is predicted between 'ymzA' and 'nrdI' PubMed
Open in new tab

nrdIymaB

2024-12-06 15:20:06

ghost

105

9d160ac30c7745496a34c93be2b3a105e0e35c39

32952B230D636C27ED0B2D37BD2B3FCE31A938B1

Description
Open in new tab

ymzAymaB

2024-12-06 18:34:49

Jstuelk

107

c327eefb1356b60d6946dc8bb1110dce66ded764

3940914A8E31A6E8EACC40EBB2BDE0A966EB921D

Biological materials
Expression vectors
pGP2894: expression of Strep-nrdF by pGP380 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
Labs working on this gene/protein
Elisabeth Härtig, Braunschweig, Germany Homepage
References
Reviews
Loading
Original Publications
Loading

CD2D14FE9C10544E7C350167EBDBD941B898051D

Page visits: 3030

Time of last update: 2024-12-12 12:22:56

Author of last update: Jstuelk