srfAA

srfAA
168

surfactin synthetase / competence

Locus
BSU_03480
Molecular weight
401.68 kDa
Isoelectric point
4.87
Protein length
Gene length
Function
antibiotic synthesis, biocontrol of fungal growth
Product
surfactin synthetase / competence
Essential
no
Synonyms
srfAA, comL

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG4908 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
376,968 → 387,731
Phenotypes of a mutant
altered cell death pattern in colonies PubMed
reduced swarming of srfAA mutant of B. subtilis NCIB3610 PubMed
defective in biofilm formation and sporulation PubMed
The protein
Protein family
3 Carrier domains (aa 971-1046, aa 2010-2085, aa 3038-3112) (according to UniProt)
Structure
2VSQ (PDB) (termination module of SrfAC, 35% identity) PubMed
Modification
phosphorylation on Ser-1006 PubMed
membrane PubMed
Additional information
the protein can be degraded by ClpC-ClpP and other adaptor-ClpP complexes PubMed
Expression and Regulation
Operons
Description
Regulation
expressed at high cell density (ComA) PubMed
heterogeneous expression, percentage of expressing cells increases during colonization of plant roots PubMed
Regulatory mechanism
PhoP: activation, PubMed, in phoP regulon
CodY: repression, PubMed, in codY regulon
ComA: activation, PubMed, in comA regulon
PerR: activation, PubMed, in perR regulon
Spx: repression, PubMed, in spx regulon
Abh: repression, PubMed, in abh regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
A ncRNA is predicted between 'HxlR' and 'SrfAA' PubMed
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srfAAsrfAD

2024-11-08 11:48:13

Jstuelk

132

620D29280C2CC5BBF61DFDE1E763667777B28513

4F5B391B1D8CC1181EDE8EB2DAC874A9B90A39CA

Additional information
the mRNA is cleaved by RNase III PubMed
Biological materials
Mutant
BKE03480 (ΔsrfAA::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATATTGTCATACCTCCCCT,  downstream forward: _UP4_GAAATTTTCTAATAGAGGTG
BKK03480 (ΔsrfAA::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATATTGTCATACCTCCCCT,  downstream forward: _UP4_GAAATTTTCTAATAGAGGTG
References
Reviews
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Original Publications
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81845F44CE2C601555066E31E87384FF5D7B4139

Page visits: 11621

Time of last update: 2024-12-04 09:42:13

Author of last update: Jstuelk