Difference between revisions of "PAC6"
| (3 intermediate revisions by 3 users not shown) | |||
| Line 1: | Line 1: | ||
[[File:PAC6.jpeg|250px|thumb|right|'''pAC6: click to enlarge''']] | [[File:PAC6.jpeg|250px|thumb|right|'''pAC6: click to enlarge''']] | ||
| − | |||
| − | |||
vector for the construction of transcriptional ''lacZ'' fusions that can be integrated at the ''amyE'' site | vector for the construction of transcriptional ''lacZ'' fusions that can be integrated at the ''amyE'' site | ||
| Line 11: | Line 9: | ||
in ''E. coli'': ampicillin resistance | in ''E. coli'': ampicillin resistance | ||
| − | in ''B. subtilis'': | + | in ''B. subtilis'': chloramphenicol resistance |
similar vectors: [[pAC5]], [[pAC7]] | similar vectors: [[pAC5]], [[pAC7]] | ||
| Line 20: | Line 18: | ||
*'''Catcheckrev:''' 5‘-GTCTGCTTTCTTCATTAGAATCAATCC-3’ | *'''Catcheckrev:''' 5‘-GTCTGCTTTCTTCATTAGAATCAATCC-3’ | ||
| − | + | <pubmed> 11902727 </pubmed> | |
| − | + | == Return to [[Plasmids]] == | |
Latest revision as of 13:53, 3 June 2013
vector for the construction of transcriptional lacZ fusions that can be integrated at the amyE site
does not replicate in B. subtilis, integrates into the amyE site
constructed in the Rapoport lab
in E. coli: ampicillin resistance
in B. subtilis: chloramphenicol resistance
sequencing primer:
- PAC5R: 5’-CTGCAAGCGATAAGTTGG-3’
- PAC5F: 5’-GCGTAGCGAAAAATCCTTTTC-3‘
- Catcheckrev: 5‘-GTCTGCTTTCTTCATTAGAATCAATCC-3’
J Stülke, I Martin-Verstraete, M Zagorec, M Rose, A Klier, G Rapoport
Induction of the Bacillus subtilis ptsGHI operon by glucose is controlled by a novel antiterminator, GlcT.
Mol Microbiol: 1997, 25(1);65-78
[PubMed:11902727]
[WorldCat.org]
[DOI]
(P p)
