rpsJ

rpsJ
168

ribosomal protein uS10

Locus
BSU_01150
Molecular weight
11.00 kDa
Isoelectric point
10
Protein length
Gene length
Function
translation
Product
ribosomal protein S10 (uS10)
Essential
yes
Synonyms
rpsJ, tetA

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0051 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
135,364  135,672
Phenotypes of a mutant
essential PubMed
The protein
Protein family
universal ribosomal protein uS10 family (single member, according to UniProt)
Structure
3R2C (PDB) (the NusB-RpsJ complex from Aquifex aeolicus) PubMed
Modification
phosphorylated on Ser-32 during germination PubMed
located near the aminoacyl-tRNA site of the 30S ribosomal subunit PubMed
Expression and Regulation
Operons
Description
Regulation
strongly repressed in response to glucose starvation in M9 medium PubMed
likely autorepression of operon expression upon binding of excess of one ribosomal protein to the untranslated region of the mRNA PubMed
expression is reduced in motile cells as compared to non-motile cells PubMed
Regulatory mechanism
stringent response: negative regulation, in stringent response
Sigma factors
SigA: sigma factor, PubMed (two promoters, about 140bp and 200bp upstream of the start codon), in sigA regulon
Additional information
term-seq has identified a potential novel regulatory RNA element (protein-dependent leader) including an intrinsic transcription terminator upstream of rpsJ PubMed
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rpsJrplQ

2024-11-12 22:53:20

Jstuelk

125

911D53E804EEFE6335D6A0F79722F9870F387CD3

9027854E648E8BF3E4A3C8492D350C69AC71C218

Biological materials
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
LacZ fusion
pGP605 (in pAC6), available in Jörg Stülke's lab
pGP2201 (in pAC7), available in Jörg Stülke's lab
pGP2439 (in pAC7), available in Jörg Stülke's lab
pGP2472 (in pAC5), available in Jörg Stülke's lab
pGP2473 (in pAC6), available in Jörg Stülke's lab
pGP2828 (in pAC6), available in Jörg Stülke's lab, only hairpin 1
pGP2829 (in pAC6), available in Jörg Stülke's lab, hairpins 1 and 2
pGP2830 (in pAC6), available in Jörg Stülke's lab, all three hairpins
References
Reviews
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Original Publications
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751D0B909922D4D931FF3B2285CF142D966FCD48

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Time of last update: 2024-12-04 01:59:09

Author of last update: Jstuelk