ymdB
168
phosphodiesterase, controls bistable gene expression, required for nanotube formation
Locus
BSU_16970
Molecular weight
29.14 kDa
Isoelectric point
6.5
Function
control of bistable gene expression
Product
phosphodiesterase
Essential
no
Synonyms
ymdB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1692 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,768,941 1,769,735
Phenotypes of a mutant
The protein
Catalyzed reaction/ biological activity
phosphodiesterase activity toward 2',3'-cAMP PubMed
nucleoside 2',3'-cyclic phosphate + H2O --> nucleoside 3'-phosphate + H+ (according to UniProt)
Structure
Expression and Regulation
Operons
Biological materials
Mutant
MGNA-B070 (ymdB::erm), available at the NBRP B. subtilis, Japan
GP1558 (ymdB::aphA3; cassette w/o terminator), available in Jörg Stülke's lab
GP1573 (ymdB::aphA3), available in Jörg Stülke's lab
Expression vectors
for expression/ purification from B. subtilis, in pBQ200: pGP1039, available in Jörg Stülke's lab
for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP1041, available in Jörg Stülke's lab
for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, in pGP382: pGP1919, available in Jörg Stülke's lab
for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP1040, available in Jörg Stülke's lab
for expression/ purification from E. coli with N-terminal Strep-tag, in pGP172: pGP1917, available in Jörg Stülke's lab PubMed
GP970 (''ymdB-Strep (cat)), purification from B. subtilis'', for SPINE, available in Jörg Stülke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
FLAG-tag construct
GP1018 (spc, based on pGP1331), available in Jörg Stülke's lab
Labs working on this gene/protein
Jörg Stülke, University of Göttingen, Germany Homepage
References
Reviews
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Original Publications
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Functional and structural analysis of orthologs in other organisms
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Time of last update: 2024-12-04 18:10:05
Author of last update: Jstuelk