Difference between revisions of "LevE"
| Line 54: | Line 54: | ||
=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' Protein EIIB N(pi)-phospho-L-histidine | + | * '''Catalyzed reaction/ biological activity:''' Protein EIIB N(pi)-phospho-L-histidine + sugar = protein EIIB + sugar phosphate (according to Swiss-Prot) |
* '''Protein family:''' [[PTS]] permease, mannose permease (Man) family [http://www.ncbi.nlm.nih.gov/pubmed/10627040 PubMed] | * '''Protein family:''' [[PTS]] permease, mannose permease (Man) family [http://www.ncbi.nlm.nih.gov/pubmed/10627040 PubMed] | ||
| Line 89: | Line 89: | ||
=Expression and regulation= | =Expression and regulation= | ||
| + | * '''Operon:''' ''[[levD]]-[[levE]]-[[levF]]-[[levG]]-[[sacC]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/2117666 PubMed] | ||
| − | * ''' | + | * '''[[Sigma factor]]:''' [[SigL]] {{PubMed|1924373}} |
| − | * '''[[ | + | * '''Regulation:''' |
| + | ** subject to carbon catabolite repression ([[CcpA]], [[LevR]]) {{PubMed|7592486}} | ||
| + | ** induced in the presence of fructose ([[LevR]]) {{PubMed|1900939}} | ||
| − | * ''' | + | * '''Regulatory mechanism:''' |
| − | + | ** [[CcpA]]: transcription repression {{PubMed|7592486}} | |
| − | * | + | ** [[LevR]]: transcription activation {{PubMed|1900939}} |
* '''Additional information:''' | * '''Additional information:''' | ||
| Line 120: | Line 123: | ||
=References= | =References= | ||
| − | <pubmed>10627040 2117666 9551099 9030753 9033408, </pubmed> | + | <pubmed>10627040 2117666 9551099 9030753 9033408,1924373 7592486 1900939</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 20:47, 25 November 2009
- Description: trigger enzyme: fructose-specific phosphotransferase system, EIIB component of the PTS
| Gene name | levE |
| Synonyms | |
| Essential | no |
| Product | trigger enzyme: fructose-specific phosphotransferase system, EIIB component |
| Function | fructose uptake and phosphorylation, control of LevR activity |
| Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism | |
| MW, pI | 17 kDa, 9.444 |
| Gene length, protein length | 486 bp, 162 aa |
| Immediate neighbours | levF, levD |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU27060
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Protein EIIB N(pi)-phospho-L-histidine + sugar = protein EIIB + sugar phosphate (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- Structure: 1BLE
- UniProt: P26380
- KEGG entry: [2]
- E.C. number: 2.7.1.69
Additional information
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
