FadH

• Description: 2,4-dienoyl-CoA reductase
  
  

Gene name	fadH
Synonyms	ykuF
Essential	no
Product	2,4-dienoyl-CoA reductase
Function	fatty acid degradation
Metabolic function and regulation of this protein in SubtiPathways: Fatty acid degradation
MW, pI	26 kDa, 6.495
Gene length, protein length	762 bp, 254 aa
Immediate neighbours	ykuE, fadG
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU14060

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

utilization of lipids

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Trans-2,3-didehydroacyl-CoA + NADP⁺ = trans,trans-2,3,4,5-tetradehydroacyl-CoA + NADPH (according to Swiss-Prot)

• Protein family: 2,4-dienoyl-CoA reductase subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O34717

• KEGG entry: [3]

• E.C. number: 1.3.1.34

Expression and regulation

• Operon: fadH-fadG PubMed

• Sigma factor:

• Regulation:
  • induced in the presence of long-chain fatty acids (FadR) PubMed

• Regulatory mechanism:
  • FadR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Yasutaro Fujita, Hiroshi Matsuoka, Kazutake Hirooka
Regulation of fatty acid metabolism in bacteria.
Mol Microbiol: 2007, 66(4);829-39
[PubMed:17919287] [WorldCat.org] [DOI] (P p)

Hiroshi Matsuoka, Kazutake Hirooka, Yasutaro Fujita
Organization and function of the YsiA regulon of Bacillus subtilis involved in fatty acid degradation.
J Biol Chem: 2007, 282(8);5180-94
[PubMed:17189250] [WorldCat.org] [DOI] (P p)