Pgi

• Description: glucose 6-phosphate isomerase, glycolytic / gluconeogenic enzyme
  
  

Gene name	pgi
Synonyms	yugL
Essential	no
Product	glucose-6-phosphate isomerase
Function	enzyme in glycolysis / gluconeogenesis
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism, Sugar catabolism
MW, pI	50.4 kDa, 4.85
Gene length, protein length	1353 bp, 451 amino acids
Immediate neighbours	yugM, yugK
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

carbon core metabolism, phosphoproteins

The gene

Basic information

• Locus tag: BSU31350

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-glucose 6-phosphate = D-fructose 6-phosphate (according to Swiss-Prot) D-glucose 6-phosphate = D-fructose 6-phosphate

• Protein family: GPI family (according to Swiss-Prot) GPI family

• Paralogous protein(s):

Extended information on the protein

• Kinetic information: Reversible Michaelis-Menten PubMed

• Domains:

• Modification: phosphorylation on Thr-39 PubMed

• Cofactor(s):

• Effectors of protein activity: competitively inhibited by 6-phosphogluconate (in B.caldotenax, B.stearothermophilus) PubMed

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot), cytoplasm

Database entries

• Structure: 1B0Z (Geobacillus stearothermophilus), 2PGI (Geobacillus stearothermophilus)

• UniProt: P80860

• KEGG entry: [3]

• E.C. number: 5.3.1.9

Additional information

• extensive information on the structure and enzymatic properties of Pgi can be found at Proteopedia

Expression and regulation

• Operon: pgi-yugM-yugN PubMed

• Sigma factor:

• Regulation: constitutively expressed PubMed

• Additional information:

Biological materials

• Mutant: GP508 (spc), available in Stülke lab

• Expression vector:
  • pGP398 (N-terminal His-tag, in pWH844), available in Stülke lab

• lacZ fusion: pGP510 (in pAC6), available in Stülke lab

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody:

Labs working on this gene/protein

Jörg Stülke, University of Göttingen, Germany homepage

References

PubMed