LevE

• Description: trigger enzyme: fructose-specific phosphotransferase system, EIIB component of the PTS
  
  

Gene name	levE
Synonyms
Essential	no
Product	trigger enzyme: fructose-specific phosphotransferase system, EIIB component
Function	fructose uptake and phosphorylation, control of LevR activity
Interactions involving this protein in SubtInteract: LevE
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	17 kDa, 9.444
Gene length, protein length	486 bp, 162 aa
Immediate neighbours	levF, levD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

phosphotransferase systems, utilization of specific carbon sources, transcription factors and their control, trigger enzyme, membrane proteins, phosphoproteins

This gene is a member of the following regulons

CcpA regulon, LevR regulon, SigL regulon

The gene

Basic information

• Locus tag: BSU27060

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein EIIB N(pi)-phospho-L-histidine + sugar = protein EIIB + sugar phosphate (according to Swiss-Prot)

• Protein family: PTS permease, mannose permease (Man) family PubMed

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: LevD-LevE, LevE-LevR

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure: 1BLE

• UniProt: P26380

• KEGG entry: [2]

• E.C. number: 2.7.1.69

Expression and regulation

• Operon: levD-levE-levF-levG-sacC PubMed

• Sigma factor: SigL PubMed

• Regulation:
  • subject to carbon catabolite repression (CcpA, LevR) PubMed
  • induced in the presence of fructose (LevR) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • LevR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: