PksS

• Description: cytochrome P450, hydroxylase of the polyketide bacillaene produced by the pks cluster
  
  

Gene name	pksS
Synonyms
Essential	no
Product	hydroxylase of the polyketide produced by the pks cluster
Function	polyketide synthesis
MW, pI	43 kDa, 6.121
Gene length, protein length	1128 bp, 376 aa
Immediate neighbours	pksR, ymzB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

miscellaneous metabolic pathways, biosynthesis of antibacterial compounds

The gene

Basic information

• Locus tag: BSU17230

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

Additional information

• A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • hydroxylation of dihydrobacillaene PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O31785

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: pksS PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Christopher T Brown, Laura K Fishwick, Binna M Chokshi, Marissa A Cuff, Jay M Jackson, Travis Oglesby, Alison T Rioux, Enrique Rodriguez, Gregory S Stupp, Austin H Trupp, James S Woollcombe-Clarke, Tracy N Wright, William J Zaragoza, Jennifer C Drew, Eric W Triplett, Wayne L Nicholson
Whole-genome sequencing and phenotypic analysis of Bacillus subtilis mutants following evolution under conditions of relaxed selection for sporulation.
Appl Environ Microbiol: 2011, 77(19);6867-77
[PubMed:21821766] [WorldCat.org] [DOI] (I p)

Jason J Reddick, Stephanie A Antolak, Gregory M Raner
PksS from Bacillus subtilis is a cytochrome P450 involved in bacillaene metabolism.
Biochem Biophys Res Commun: 2007, 358(1);363-7
[PubMed:17482575] [WorldCat.org] [DOI] (P p)