PonA

• Description: penicillin-binding protein 1A/1B
  
  

Gene name	ponA
Synonyms
Essential	no
Product	penicillin-binding protein 1A/1B
Function	bifunctional glucosyltransferase/ transpeptidase
Gene expression levels in SubtiExpress: ponA
MW, pI	99 kDa, 4.752
Gene length, protein length	2742 bp, 914 aa
Immediate neighbours	recU, ypoC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins

This gene is a member of the following regulons

SigM regulon

The gene

Basic information

• Locus tag: BSU22320

Phenotypes of a mutant

• prevents bulging of the cells when grown at low Mg(2+) concentrations, suppresses the lethal effect of a mreB mutation PubMed
• deletion of ponA restores growth and normal shape of a yvcK mutant on gluconeogenic carbon sources PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • membrane associated PubMed
  • localizes to the spore septum during sporulation PubMed
  • localizes to the vegetative septum PubMed, this depends on YvcK PubMed

Database entries

• Structure:

• UniProt: P39793

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: recU-ponA PubMed

• Expression browser: ponA PubMed

• Sigma factor: SigA PubMed, SigM PubMed

• Regulation: constitutive during vegetative growth PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jeff Errington lab

• Antibody:

Labs working on this gene/protein

Jeff Errington, Newcastle University, UK homepage

References

Reviews

Johann Mignolet, Patrick H Viollier
A sweet twist gets Bacillus into shape.
Mol Microbiol: 2011, 80(2);283-5
[PubMed:21371139] [WorldCat.org] [DOI] (I p)