GanA

• Description: beta-galactosidase
  
  

Gene name	ganA
Synonyms	yvfN, lacA
Essential	no
Product	beta-galactosidase
Function	galactan utilization
Gene expression levels in SubtiExpress: ganA
MW, pI	79 kDa, 5.704
Gene length, protein length	2061 bp, 687 aa
Immediate neighbours	ganB, ganQ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources, phosphoproteins

This gene is a member of the following regulons

LacR regulon

The gene

Basic information

• Locus tag: BSU34130

Phenotypes of a mutant

• a cycB-ganP-ganQ-ganA-ganB mutant is impaired in the utilization of galactan PubMed

Database entries

• BsubCyc: BSU34130

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • degradation of galactotriose to galactose PubMed

• Protein family: glycosyl hydrolase 42 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-387 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU34130

• Structure:

• UniProt: O07012

• KEGG entry: [3]

• E.C. number: 3.2.1.23 3

Expression and regulation

• Operon: cycB-ganP-ganQ-ganA-ganB PubMed

• Expression browser: ganA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:
  • LacR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:GP1181 (aphA3) available in the Stülke lab
  • 1A785 ( ganA::spec), PubMed, available at BGSC
  • 1A786 ( ganA::spec), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: