DctP

• Description: uptake of succinate, fumurate, malate and oxaloacetate via proton symport
  
  

Gene name	dctP
Synonyms	ydbH, dctA
Essential	no
Product	C4-dicarboxylate transport protein
Function	uptake of succinate, fumurate, malate and oxaloacetate
Gene expression levels in SubtiExpress: dctP
Interactions involving this protein in SubtInteract: DctP
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	45 kDa, 8.668
Gene length, protein length	1263 bp, 421 aa
Immediate neighbours	dctR, ydbI
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, utilization of specific carbon sources, membrane proteins

This gene is a member of the following regulons

CcpA regulon, FsrA regulon

The gene

Basic information

• Locus tag: BSU04470

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • uptake of succinate, fumurate, malate and oxaloacetate via proton symport PubMed
  • acts as co-sensor for DctS PubMed

• Protein family: View classification (according to Swiss-Prot)

• Paralogous protein(s): GltP, GltT

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • DctB-DctS-DctP, sensing complex PubMed

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure: 4KY0 (the glutamate transporter of Thermococcus kodakarensis, 31% identity, 71% similarity) PubMed

• UniProt: P96603

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:
  • dctS-dctR-dctP PubMed
  • dctP PubMed

• Expression browser: dctP PubMed

• Sigma factor:
  • dctP: SigA PubMed

• Regulation:
  • repressed by glucose (6.2-fold) (CcpA) PubMed
  • part of the iron sparing response, strong down-regulation in a fur mutant (Fur, FsrA) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • Fur: indirect effect via control of fsrA expression PubMed
  • FsrA: translation inhibition PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Sabrina Graf, Dominik Schmieden, Karolin Tschauner, Sabine Hunke, Gottfried Unden
The sensor kinase DctS forms a tripartite sensor unit with DctB and DctA for sensing C4-dicarboxylates in Bacillus subtilis.
J Bacteriol: 2014, 196(5);1084-93
[PubMed:24375102] [WorldCat.org] [DOI] (I p)

Sonja Jensen, Albert Guskov, Stephan Rempel, Inga Hänelt, Dirk Jan Slotboom
Crystal structure of a substrate-free aspartate transporter.
Nat Struct Mol Biol: 2013, 20(10);1224-6
[PubMed:24013209] [WorldCat.org] [DOI] (I p)

Bogumiła C Marciniak, Monika Pabijaniak, Anne de Jong, Robert Dűhring, Gerald Seidel, Wolfgang Hillen, Oscar P Kuipers
High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis.
BMC Genomics: 2012, 13;401
[PubMed:22900538] [WorldCat.org] [DOI] (I e)

Gregory T Smaldone, Olga Revelles, Ahmed Gaballa, Uwe Sauer, Haike Antelmann, John D Helmann
A global investigation of the Bacillus subtilis iron-sparing response identifies major changes in metabolism.
J Bacteriol: 2012, 194(10);2594-605
[PubMed:22389480] [WorldCat.org] [DOI] (I p)

Maarten Groeneveld, Ruud G J Detert Oude Weme, Ria H Duurkens, Dirk Jan Slotboom
Biochemical characterization of the C4-dicarboxylate transporter DctA from Bacillus subtilis.
J Bacteriol: 2010, 192(11);2900-7
[PubMed:20363944] [WorldCat.org] [DOI] (I p)

Kousei Tanaka, Kazuo Kobayashi, Naotake Ogasawara
The Bacillus subtilis YufLM two-component system regulates the expression of the malate transporters MaeN (YufR) and YflS, and is essential for utilization of malate in minimal medium.
Microbiology (Reading): 2003, 149(Pt 9);2317-2329
[PubMed:12949159] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)

Kei Asai, Sang-Hoon Baik, Yasuhiro Kasahara, Shigeki Moriya, Naotake Ogasawara
Regulation of the transport system for C4-dicarboxylic acids in Bacillus subtilis.
Microbiology (Reading): 2000, 146 ( Pt 2);263-271
[PubMed:10708364] [WorldCat.org] [DOI] (P p)

Ralf Rabus, Donald L Jack, David J Kelly, Milton H Saier
TRAP transporters: an ancient family of extracytoplasmic solute-receptor-dependent secondary active transporters.
Microbiology (Reading): 1999, 145 ( Pt 12);3431-3445
[PubMed:10627041] [WorldCat.org] [DOI] (P p)