SacY

• Description: transcriptional antiterminator for sacB and sacX-sacY (acts at high sucrose concentrations)
  
  

Gene name	sacY
Synonyms	ipa-13r, sacS
Essential	no
Product	transcriptional antiterminator
Function	regulation of sucrose utilization
Gene expression levels in SubtiExpress: sacY
Interactions involving this protein in SubtInteract: SacY
Metabolic function and regulation of this protein in SubtiPathways: sacY
MW, pI	32 kDa, 5.883
Gene length, protein length	840 bp, 280 aa
Immediate neighbours	sacX, gspA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources, transcription factors and their control, RNA binding regulators, phosphoproteins

This gene is a member of the following regulons

SacY regulon

The gene

Basic information

• Locus tag: BSU38420

Database entries

• BsubCyc: BSU38420

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: binding to the mRNA of sacB and the sacX-sacY operon, causes transcription antitermination (in presence of sucrose)

• Protein family: transcriptional antiterminator bglG family (according to Swiss-Prot) BglG family of antiterminators

• Paralogous protein(s): LicT, GlcT, SacT

Extended information on the protein

• Kinetic information:
  • K(D) for the RAT-RNA: 3 nM PubMed
• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • PtsH-SacY

• Localization:

Database entries

• BsubCyc: BSU38420

• Structure: 1AUU (RNA-binding domain)

• UniProt: P15401

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: sacX-sacY PubMed

• Expression browser: sacY PubMed

• Sigma factor: SigA PubMed

• Regulation: induction by sucrose (at high concentration) PubMed

• Regulatory mechanism:
  • induction by SacY-dependent RNA switch (transcriptional antitermination) PubMed
  • DegU-P: transcription activation PubMed

• Additional information:

Biological materials

• Mutant: GP425 (cat), available in Stülke lab

• Expression vector:
  • for expression, purification of the RNA-binding domain in E. coli with N-terminal His-tag, in pWH844: pGP316, available in Stülke lab
  • for expression, purification of the RNA-binding domain in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP573, available in Stülke lab

• lacZ fusion:

• GFP fusion: GP1226 (spc, based on pGP1870), available in the Stülke lab

• YFP fusion: GP1230 (spc, based on pGP1871), available in the Stülke lab

• FLAG-tag construct: GP1222 (spc, based on pGP1331), available in the Stülke lab

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France