LmrB

• Description: lincomycin-resistance protein (multidrug resistance pump)
  
  

Gene name	lmrB
Synonyms	yccA
Essential	no
Product	lincomycin-resistance protein
Function	resistance to lincomycin
Gene expression levels in SubtiExpress: lmrB
MW, pI	51 kDa, 9.703
Gene length, protein length	1437 bp, 479 aa
Immediate neighbours	ycbU, lmrA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, resistance against toxins/ antibiotics, membrane proteins

This gene is a member of the following regulons

LmrA regulon

The gene

Basic information

• Locus tag: BSU02670

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: EmrB family (according to Swiss-Prot)

• Paralogous protein(s): YcnB, YhcA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O35018

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: lmrA-lmrB PubMed

• Expression browser: lmrB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by flavonoids such as quercetin (LmrA)PubMed

• Regulatory mechanism:
  • LmrA: transcription repression PubMed

• Additional information:
  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

• Mutant:
  • GP1701 (lmrB::aphA3), available in Jörg Stülke's lab
  • GP1703 (lmrB::aphA3 ycnB::spc), available in Jörg Stülke's lab
• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: