SunA

• Description: sublancin 168 lantibiotic antimicrobial precursor peptide
  
  

Gene name	sunA
Synonyms	yolG
Essential	no
Product	sublancin 168 lantibiotic antimicrobial precursor peptide
Function	antimicrobial peptide
Gene expression levels in SubtiExpress: sunA
MW, pI	5 kDa, 7.963
Gene length, protein length	168 bp, 56 aa
Immediate neighbours	sunT, sunI
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

miscellaneous metabolic pathways, biosynthesis of antibacterial compounds, toxins, antitoxins and immunity against toxins, SP-beta prophage, membrane proteins

This gene is a member of the following regulons

Abh regulon, AbrB regulon, Rok regulon, YvrHb regulon

The gene

Basic information

• Locus tag: BSU21480

Database entries

• BsubCyc: BSU21480

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: IPP isomerase type 2 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: contains a glucose attached to a cysteine residue, glycosylation is essential for its antimicrobial activity PubMed

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU21480

• Structure: 2MIJ PubMed

• UniProt: P68577

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: sunA PubMed

• Expression browser: sunA PubMed

• Sigma factor:

• Regulation:
  • repressed by Rok PubMed
  • repressed during logrithmic growth (AbrB) PubMed

• Regulatory mechanism:
  • activated by YvrHb PubMed
  • Rok: transcription repression PubMed
  • AbrB: transcription repressionPubMed
  • Abh: transcription activation PubMed

• Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:
  • GP1563 (aphA3), available in Jörg Stülke's lab
  • GP1565 (sunA-sunI, aphA3), available in Jörg Stülke's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: