NadF

• Description: NAD kinase
  
  

Gene name	nadF
Synonyms	yjbN, ppnK
Essential	yes PubMed
Product	inorganic polyphosphate/ATP-NAD kinase
Function	generation of NADP from NAD
Gene expression levels in SubtiExpress: nadF
Metabolic function and regulation of this protein in SubtiPathways: NadF
MW, pI	29 kDa, 5.991
Gene length, protein length	798 bp, 266 aa
Immediate neighbours	sasB, yjbO
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors, essential genes

The gene

Basic information

• Locus tag: BSU11610

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU11610

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + NAD⁺ = ADP + NADP⁺ (according to Swiss-Prot)

• Protein family: NAD kinase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity: allosterically activated by quinolinic acid

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU11610

• Structure:

• UniProt: O31612

• KEGG entry: [2]

• E.C. number: 2.7.1.23

Expression and regulation

• Operon: yjbL-sasB-nadF-yjbO PubMed

• Expression browser: nadF PubMed

• Sigma factor:

• Regulation: expressed during exponential growth

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 88 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 405 PubMed

Biological materials

• Mutant:
  • 1A825 ( nadF::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: