RpoC

• Description: RNA polymerase beta' subunit
  
  

Gene name	rpoC
Synonyms
Essential	yes PubMed
Product	RNA polymerase beta' subunit
Function	transcription
Gene expression levels in SubtiExpress: rpoC
Interactions involving this protein in SubtInteract: RpoC
MW, pI	133 kDa, 8.863
Gene length, protein length	3597 bp, 1199 aa
Immediate neighbours	rpoB, ybxF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription, essential genes, membrane proteins, phosphoproteins

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU01080

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU01080

• DBTBS entry: no entry

• SubtiList entry: [1]

Additional information

• mutations in mtrB, sigB, rpoB, and rpoC allow B. subtilis to grow with 4-fluorotryptophan rather than with tryptophan as a canonical amino acid of the genetic code PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1) (according to Swiss-Prot)

• Protein family: RNA polymerase beta' chain family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-335 and Arg-803 PubMed

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • RpoA-RpoB-RpoC PubMed
  • RpoC-RpoY PubMed
  • SigA-(RpoB-RpoC) PubMed, SigB-(RpoB-RpoC)
  • SigD-(RpoB-RpoC), SigE-(RpoB-RpoC)
  • SigF-(RpoB-RpoC), SigG-(RpoB-RpoC)
  • SigH-(RpoB-RpoC), SigI-(RpoB-RpoC)
  • SigK-(RpoB-RpoC), SigL-(RpoB-RpoC)
  • SigM-(RpoB-RpoC), SigV-(RpoB-RpoC)
  • SigW-(RpoB-RpoC), SigX-(RpoB-RpoC)
  • SigY-(RpoB-RpoC), SigZ-(RpoB-RpoC)
  • Xpf-(RpoB-RpoC), YlaC-(RpoB-RpoC)
  • YvrHa-RpoC PubMed
  • RpoC-RpoE (demonstrated for S. aureus) PubMed

• Localization:
  • colocalizes with the nucleoid PubMed
  • membrane associated PubMed

Database entries

• BsubCyc: BSU01080

• Structure:

• UniProt: P37871

• KEGG entry: [2]

• E.C. number: 2.7.7.6

Expression and regulation

• Operon: rpoB-rpoC PubMed

• Expression browser: rpoC PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 2487 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 8264 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab

• Antibody:

References

Reviews

Nikolay Zenkin
Multiple personalities of the RNA polymerase active centre.
Microbiology (Reading): 2014, 160(Pt 7);1316-1320
[PubMed:24763425] [WorldCat.org] [DOI] (I p)

Lakshminarayan M Iyer, L Aravind
Insights from the architecture of the bacterial transcription apparatus.
J Struct Biol: 2012, 179(3);299-319
[PubMed:22210308] [WorldCat.org] [DOI] (I p)