KtrA

• Description: high affinity potassium transporter KtrA-KtrB, peripheric membrane component (proton symport)
  
  

Gene name	ktrA
Synonyms	yuaA
Essential	no
Product	high affinity potassium transporter KtrA-KtrB, peripheric membrane component (proton symport)
Function	potassium uptake
Gene expression levels in SubtiExpress: ktrA
Interactions involving this protein in SubtInteract: KtrA
Metabolic function and regulation of this protein in SubtiPathways: Metal ion homeostasis, ktrA
MW, pI	24 kDa, 5.981
Gene length, protein length	666 bp, 222 aa
Immediate neighbours	bslA, ktrB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, metal ion homeostasis (K, Na, Ca, Mg), coping with hyper-osmotic stress, membrane proteins

This gene is a member of the following regulons

YdaO riboswitch

The gene

Basic information

• Locus tag: BSU31090

Phenotypes of a mutant

• The ktrA-ktrB mutant of B. subtilis NCIB3610 is reduced in sliding (dendritic spreading) PubMed

Database entries

• BsubCyc: BSU31090

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): KtrC

Extended information on the protein

• Kinetic information:

• Domains:
  • contains a RCK_N domain at the N-terminus (according to UniProt, [2])
  • contains a RCK_C domain at the C-terminus (according to UniProt, [3])

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • KtrA-KtrB PubMed, composed of an octameric KtrA ring and two KtrB dimers PubMed

• Localization: peripheral membrane protein PubMed

Database entries

• BsubCyc: BSU31090

• Structure:
  • 1LSU (complex with NADH)
  • 2HMW ( complex with ATP)
  • 4J7C (the KtrA-KtrB complex) PubMed

• UniProt: O32080

• KEGG entry: [4]

• E.C. number:

Expression and regulation

• Operon: ktrA-ktrB PubMed

• Expression browser: ktrA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:
  • expression is controlled via termination antitermination by the ydaO riboswitch, expression is switched off upon binding of c-di-AMP PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A954 ( ktrA::kan), PubMed, available at BGSC
  • GHB1 (D(ktrA-ktrB)::aphA3), available in Erhard Bremer's lab
  • GP92 (D(ktrA-ktrB)::aphA3), available in Jörg Stülke's lab

• Expression vector:
  • pGP2594: (IPTG inducible expression, purification in E. coli with N-terminal His-tag, in pWH844), available in Jörg Stülke's lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Erhard Bremer, University of Marburg, Germany homepage

References

Reviews

Fabian M Commichau, Achim Dickmanns, Jan Gundlach, Ralf Ficner, Jörg Stülke
A jack of all trades: the multiple roles of the unique essential second messenger cyclic di-AMP.
Mol Microbiol: 2015, 97(2);189-204
[PubMed:25869574] [WorldCat.org] [DOI] (I p)