Difference between revisions of "AcsA"
| Line 89: | Line 89: | ||
=Expression and regulation= | =Expression and regulation= | ||
| + | * '''Operon:''' ''[[acsA]]'' {{PubMed|7934817}} | ||
| − | * ''' | + | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|7913927}} |
| − | * '''[[ | + | * '''Regulation:''' |
| + | ** repressed by glucose (4.5-fold) ([[CcpA]]) {{PubMed|7913927,12850135}} | ||
| + | ** repressed during growth in the presence of branched chain amino acids ([[CodY]]) {{PubMed|12618455,18083814}} | ||
| − | * ''' | + | * '''Regulatory mechanism:''' |
| − | ** | + | ** [[CcpA]]: transcription repression {{PubMed|7913927}} |
| − | + | ** [[CodY]]: transcription repression {{PubMed|12618455,18083814}} | |
| − | |||
| − | ** [[CodY]]: transcription repression | ||
* '''Additional information:''' | * '''Additional information:''' | ||
Revision as of 11:35, 19 December 2009
- Description: acetyl-CoA synthetase
| Gene name | acsA |
| Synonyms | |
| Essential | no |
| Product | acetyl-CoA synthetase) |
| Function | utilization of acetate, fatty acids |
| Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
| MW, pI | 64 kDa, 5.547 |
| Gene length, protein length | 1716 bp, 572 aa |
| Immediate neighbours | tyrS, acuA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU29680
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA (according to Swiss-Prot)
- Protein family: ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification: acetylated on Lys-549 by AcuA, this results in inactivation PubMed, deacetylated by SrtN and AcuC deacetylates (and thereby activates) AcsA PubMed
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: P39062
- KEGG entry: [3]
- E.C. number: 6.2.1.1
Additional information
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
