Difference between revisions of "EpsE"
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| − | * '''Description:''' inhibitor of motility <br/><br/> | + | * '''Description:''' inhibitor of motility and glycosyltransferase required for EPS biosynthesis <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Product''' || inhibitor of motility | + | |style="background:#ABCDEF;" align="center"| '''Product''' || glycosyltransferase, inhibitor of motility |
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || [[biofilm formation]] | |style="background:#ABCDEF;" align="center"|'''Function''' || [[biofilm formation]] | ||
| Line 69: | Line 69: | ||
* '''Catalyzed reaction/ biological activity:''' | * '''Catalyzed reaction/ biological activity:''' | ||
** arrests flagellar rotation in a manner similar to that of a clutch, by disengaging motor force-generating elements in cells embedded in the biofilm matrix {{PubMed|18566286}} | ** arrests flagellar rotation in a manner similar to that of a clutch, by disengaging motor force-generating elements in cells embedded in the biofilm matrix {{PubMed|18566286}} | ||
| + | ** biosynthesis of extracellular polysaccharides {{PubMed| 21170308}} | ||
* '''Protein family:''' glycosyltransferase 2 family (according to Swiss-Prot) | * '''Protein family:''' glycosyltransferase 2 family (according to Swiss-Prot) | ||
| Line 142: | Line 143: | ||
<pubmed>20735481 </pubmed> | <pubmed>20735481 </pubmed> | ||
==Original publications== | ==Original publications== | ||
| − | <pubmed>15661000,16430695,18047568, 18566286 18647168 20817675</pubmed> | + | <pubmed>15661000,16430695,18047568, 18566286 18647168 20817675 21170308 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 10:33, 21 December 2010
- Description: inhibitor of motility and glycosyltransferase required for EPS biosynthesis
| Gene name | epsE |
| Synonyms | yveO |
| Essential | no |
| Product | glycosyltransferase, inhibitor of motility |
| Function | biofilm formation |
| Regulation of this protein in SubtiPathways: Biofilm | |
| MW, pI | 32 kDa, 9.804 |
| Gene length, protein length | 834 bp, 278 aa |
| Immediate neighbours | epsF, epsD |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
motility and chemotaxis, biofilm formation, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU34330
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- arrests flagellar rotation in a manner similar to that of a clutch, by disengaging motor force-generating elements in cells embedded in the biofilm matrix PubMed
- biosynthesis of extracellular polysaccharides 21170308 PubMed
- Protein family: glycosyltransferase 2 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cell membrane, forms spots at flagellar basal bodies PubMed
Database entries
- Structure:
- UniProt: P71054
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
- Daniel Kearns, Indiana University, Bloomington, USA, homepage
- Richard Losick, Harvard Univ., Cambridge, USA homepage
Your additional remarks
References
Reviews
Massimiliano Marvasi, Pieter T Visscher, Lilliam Casillas Martinez
Exopolymeric substances (EPS) from Bacillus subtilis: polymers and genes encoding their synthesis.
FEMS Microbiol Lett: 2010, 313(1);1-9
[PubMed:20735481]
[WorldCat.org]
[DOI]
(I p)
Original publications
