Difference between revisions of "RnpA"
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| − | * '''Description:''' protein component of [[RNase]] P, metal-dependent 5' end maturation of precursor [[ | + | * '''Description:''' protein component of [[RNase]] P, metal-dependent 5' end maturation of precursor [[tRNA]]s <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
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|style="background:#ABCDEF;" align="center"| '''Product''' || protein component of [[RNase]] P (substrate specificity) | |style="background:#ABCDEF;" align="center"| '''Product''' || protein component of [[RNase]] P (substrate specificity) | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Function''' || cleavage of precursor sequences <br/>from the 5' ends of pre-[[ | + | |style="background:#ABCDEF;" align="center"|'''Function''' || cleavage of precursor sequences <br/>from the 5' ends of pre-[[tRNA]]s |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 13 kDa, 10.804 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 13 kDa, 10.804 | ||
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=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor (according to Swiss-Prot) | + | * '''Catalyzed reaction/ biological activity:''' Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from [[tRNA]] precursor (according to Swiss-Prot) |
* '''Protein family:''' rnpA family (according to Swiss-Prot) | * '''Protein family:''' rnpA family (according to Swiss-Prot) | ||
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* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
| − | * '''Interactions:''' [[RnpA]]-[[RpmH]] | + | * '''[[Interactions]]:''' |
| + | ** [[RnpA]]-[[RpmH]] | ||
| + | ** [[RnpA]]-[[RnpB]] {{PubMed|21622899}} | ||
* '''Localization:''' | * '''Localization:''' | ||
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* '''Structure:''' | * '''Structure:''' | ||
** [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1A6F 1AF6] [http://www.ncbi.nlm.nih.gov/sites/entrez/9563955 PubMed] | ** [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1A6F 1AF6] [http://www.ncbi.nlm.nih.gov/sites/entrez/9563955 PubMed] | ||
| − | ** [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=3OKB 3OKB] (complex of RnpA with the RNA RnpB and tRNA, from ''Thermotoga maritima'') {{PubMed|21076397}} | + | ** [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=3OKB 3OKB] (complex of RnpA with the RNA [[RnpB]] and [[tRNA]], from ''Thermotoga maritima'') {{PubMed|21076397}} |
* '''UniProt:''' [http://www.uniprot.org/uniprot/P25814 P25814] | * '''UniProt:''' [http://www.uniprot.org/uniprot/P25814 P25814] | ||
Revision as of 08:29, 2 June 2011
| Gene name | rnpA |
| Synonyms | |
| Essential | yes PubMed |
| Product | protein component of RNase P (substrate specificity) |
| Function | cleavage of precursor sequences from the 5' ends of pre-tRNAs |
| MW, pI | 13 kDa, 10.804 |
| Gene length, protein length | 348 bp, 116 aa |
| Immediate neighbours | spoIIIJ, rpmH |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
Rnases, translation, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU41050
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor (according to Swiss-Prot)
- Protein family: rnpA family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- Structure:
- UniProt: P25814
- KEGG entry: [2]
- E.C. number: 3.1.26.5
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
- Carol Fierke, University of Michigan, Ann Arbor, USA homepage
- Roland Hartmann, Marburg University, Germany homepage
Your additional remarks
References
Reviews
Original Publications
Additional publications: PubMed
