Difference between revisions of "CysC"

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Revision as of 09:15, 19 April 2012

  • Description: adenylyl-sulfate kinase

Gene name cysC
Synonyms ylnC
Essential no
Product adenylyl-sulfate kinase
Function sulfate reduction and activation
Metabolic function and regulation of this protein in SubtiPathways:
Cys, Met & Sulfate assimilation
MW, pI 22 kDa, 5.083
Gene length, protein length 591 bp, 197 aa
Immediate neighbours sat, ylnD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
CysC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CysC expression.png




























Categories containing this gene/protein

sulfur metabolism

This gene is a member of the following regulons

CymR regulon, S-box

The gene

Basic information

  • Locus tag: BSU15600

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate (according to Swiss-Prot)
  • Protein family: APS kinase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3CR8 (from Thiobacillus denitrificans, 46% identity, 62% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • repressed by casamino acids PubMed
    • repressed in the presence of cysteine (CymR)
    • induced by methionine starvation (S-box) PubMed
  • Regulatory mechanism:
    • S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed
    • CymR: transcription repression
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Isabelle Martin-Verstraete, Institute Pasteur, Paris, France

Your additional remarks

References